ATCC BAA - 365中自溶相关基因的鉴定与功能验证 - Suppr

Identification and Functional Validation of Autolysis-Associated Genes in ATCC BAA-365.

作者信息

Pang Xiaoyang, Zhang Shuwen, Lu Jing, Liu Lu, Ma Changlu, Yang Yang, Ti Panpan, Gao Weihua, Lv Jiaping

机构信息

Key Laboratory of Agro-Food Processing and Quality Control, Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural ScienceBeijing, China.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business UniversityBeijing, China.

出版信息

Front Microbiol. 2017 Jul 19;8:1367. doi: 10.3389/fmicb.2017.01367. eCollection 2017.

Lactic acid bacteria (LAB) are important organisms in food production. Indeed, LAB autolysis is very critical in dairy processing. For example, it influences the development of cheese flavor by releasing intracellular enzymes, and controls cell growth in yogurts and probiotic products. Two component systems (TCS) constitute essential environmental sensors and effectors of signal transduction in most bacteria. In the present work, mutants of one TCS (LBUL_RS00115/LBUL_RS00110) were generated to assess the relationship between TCS and cell autolysis. The mutants displayed decreased autolysis in comparison with wild type; meanwhile, complementation reversed this effect. The interaction between LBUL_RS00115 and LBUL_RS00110 was confirmed by yeast two-hybrid analysis. These observations suggested that the TCS (LBUL_RS00115/LBUL_RS00110) was involved in autolysis in subsp. .

乳酸菌（LAB）是食品生产中的重要微生物。事实上，LAB自溶在乳制品加工中非常关键。例如，它通过释放细胞内酶影响奶酪风味的形成，并控制酸奶和益生菌产品中的细胞生长。双组分系统（TCS）是大多数细菌中重要的环境传感器和信号转导效应器。在本研究中，构建了一个TCS（LBUL_RS00115/LBUL_RS00110）的突变体，以评估TCS与细胞自溶之间的关系。与野生型相比，突变体的自溶能力下降；同时，互补作用逆转了这种效应。通过酵母双杂交分析证实了LBUL_RS00115和LBUL_RS00110之间的相互作用。这些观察结果表明，TCS（LBUL_RS00115/LBUL_RS00110）参与了亚种的自溶过程。