Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science and Technology Normal University, No. 605, Feng Lin Road, Nanchang, 330013, China.
School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100016, China.
Arch Pharm Res. 2018 Dec;41(12):1190-1198. doi: 10.1007/s12272-017-0941-y. Epub 2017 Aug 2.
Previous studies have shown that flavonoids (Fs) present in Linaria vulgaris inhibit lipid accumulation in vitro. This study was designed to evaluate the effects of Fs extracted from Linaria vulgaris ssp. sinensis (Bebeaux) Hong, on hyperlipidemia and hepatic steatosis induced by a western-type diet in mice. The major constituents of Fs were analyzed by LC-MS analysis. C57BL/6 mice were fed a western-type diet for 8 weeks to induce hyperlipidemia (model group), or fed a western-type diet followed by Fs treatment (90, 30 or 10 mg/kg/day) or atorvastatin treatment (1.0 mg/kg/day), for 8 weeks. It was found that Fs treatment resulted in significant reductions in serum levels of AST, ALT, TC, TG, LDL-C, free fatty acid and hepatic TC, and TG compared to those in model mice with hyperlipidemia (P < 0.05). The mice treated with Fs showed a relatively normal hepatic architecture compared to the hepatic steatosis shown in the model group. Moreover, the expressions of mature forms of sterol regulatory element-binding proteins (nuclear form of srebps, n-SREBPs) and 3-hydroxy-3-methylglutaryl coenzyme reductase (HMGCR) involved in lipid metabolism, were suppressed in the Fs-treated groups. Taken together, these results suggest Fs exert protective effects against hyperlipidemia and hepatic steatosis, which may involve the inhibition of mature SREBPs expressions.
先前的研究表明，普通庭荠中存在的类黄酮（Fs）可抑制体外脂质积累。本研究旨在评估从中华庭荠（Bebeaux）Hong 亚种中提取的 Fs 对西式饮食诱导的小鼠高脂血症和肝脂肪变性的影响。通过 LC-MS 分析对 Fs 的主要成分进行了分析。用西式饮食喂养 C57BL/6 小鼠 8 周，以诱导高脂血症（模型组），或在用西式饮食喂养后用 Fs 处理（90、30 或 10mg/kg/天）或阿托伐他汀处理（1.0mg/kg/天），持续 8 周。结果发现，与高脂血症模型小鼠相比，Fs 处理可显著降低血清 AST、ALT、TC、TG、LDL-C、游离脂肪酸和肝 TC、TG 水平（P<0.05）。与模型组的肝脂肪变性相比，用 Fs 处理的小鼠肝组织形态学相对正常。此外，参与脂质代谢的固醇调节元件结合蛋白的成熟形式（核形式的 SREBPs，n-SREBPs）和 3-羟基-3-甲基戊二酰基辅酶 A 还原酶（HMGCR）的表达在 Fs 处理组中受到抑制。综上所述，这些结果表明 Fs 对高脂血症和肝脂肪变性具有保护作用，这可能涉及抑制成熟 SREBPs 的表达。
