Xiao Lin, Jia Hai-Fei, Jeong In-Hong, Ahn Young-Joon, Zhu Yong-Zhe
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University , Changcheng Road, Chengyang District, Qingdao, Shandong 266-109, China.
Division of Crop Protection, National Institute of Agricultural Science, Rural Development Administration , Jeonju 55365, Jeollabuk-do Republic of Korea.
J Agric Food Chem. 2017 Aug 30;65(34):7345-7351. doi: 10.1021/acs.jafc.7b02140. Epub 2017 Aug 17.
Strain ZX02 was isolated from Chinese ginger cultivated soil contaminated with various pesticides, which could utilize 2,4-dichlorophenoxyacetic acid butyl ester (2,4-D butyl ester) as the sole carbon source. On the basis of the sequence analysis of 16S rRNA gene as well as the morphological, biochemical, and physiological characteristics of strain ZX02, the organism belonged to Gram-negative bacterium and was identified as Acinetobacter sp. ZX02. The strain ZX02 showed a remarkable performance in 2,4-D butyl ester degradation (100% removal in <96 h) in pure culture. Strain ZX02 was sensitive to tetracycline and resistant to amoxicillin and chloramphenicol in an antibiotic sensitivity test. The curing study indicates that the gene for degradation of 2,4-D butyl ester was encoded on a single plasmid of 23 kb. The gene encoding resistance to polymixin B sulfate was also located on this plasmid. On the basis of its greater biodegradation activity, this bacterium is a potential candidate as a bioremediation agent in soils contaminated with 2,4-D butyl ester.
菌株ZX02是从受多种农药污染的中国生姜种植土壤中分离得到的，它能够利用2,4-二氯苯氧乙酸丁酯（2,4-D丁酯）作为唯一碳源。基于16S rRNA基因序列分析以及菌株ZX02的形态、生化和生理特征，该菌株属于革兰氏阴性菌，被鉴定为不动杆菌属ZX02。菌株ZX02在纯培养中对2,4-D丁酯的降解表现出显著性能（在<96小时内100%去除）。在抗生素敏感性试验中，菌株ZX02对四环素敏感，对阿莫西林和氯霉素耐药。消除研究表明，2,4-D丁酯降解基因编码在一个23 kb的单一质粒上。编码对硫酸多粘菌素B耐药性的基因也位于该质粒上。基于其较强的生物降解活性，该细菌是受2,4-D丁酯污染土壤生物修复剂的潜在候选者。
