Department of Thoracic Surgery, The Second Hospital of Tianjin Medical University, Tianjin, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.
Thorac Cancer. 2017 Nov;8(6):572-576. doi: 10.1111/1759-7714.12479. Epub 2017 Aug 3.
This study examined the expression of exchange protein directly activated by cAMP1 (Epac1), PDE4, and PKC in esophageal cancer tissues, and analyzed the association of each protein with the pathological parameters of the samples.
Epac1, PDE4, and PKC protein expression was evaluated by PV-9000 two-step immunohistochemical techniques in 51 esophageal cancer specimens and 10 para-carcinoma tissues.
The positive expression rates of Epac1 and PKC in esophageal cancer tissues (62.7% and 68.6%, respectively) were higher compared to those in para-carcinoma tissues (20% and 20%, respectively) (P < 0.05). The positive expression rate of PDE4 in esophageal cancer tissues (54.1%) was higher than in para-carcinoma tissues (30%), (P > 0.05). Epac1, PDE4, and PKC protein expression levels were not associated with the extent of tumor differentiation and/or lymph node metastasis (P > 0.05). Epac1 protein expression levels correlated with PDE4, PKC, and AKAP95 protein expression levels. In addition, there was a correlation between PKC and Cx43 protein levels (P < 0.05).
The expression rates of Epac1, PDE4, and PKC protein in esophageal cancer tissues were significantly higher compared to the rates in para-carcinoma tissues, suggesting an association between these proteins and the development and progression of esophageal cancer. The correlations between these proteins also revealed that they may exert a synergistic effect during the development of esophageal cancer.
本研究检测了交换蛋白直接激活环 AMP1（Epac1）、PDE4 和蛋白激酶 C 在食管癌组织中的表达，并分析了每种蛋白与样本病理参数的关系。
采用 PV-9000 两步免疫组化技术检测 51 例食管癌标本和 10 例癌旁组织中 Epac1、PDE4 和 PKC 蛋白的表达。
食管癌组织中 Epac1 和 PKC 的阳性表达率（分别为 62.7%和 68.6%）高于癌旁组织（分别为 20%和 20%）（P<0.05）。食管癌组织中 PDE4 的阳性表达率（54.1%）高于癌旁组织（30%）（P>0.05）。Epac1、PDE4 和 PKC 蛋白表达水平与肿瘤分化程度和/或淋巴结转移无相关性（P>0.05）。Epac1 蛋白表达水平与 PDE4、PKC 和 AKAP95 蛋白表达水平相关，此外 PKC 与 Cx43 蛋白水平相关（P<0.05）。
食管癌组织中 Epac1、PDE4 和 PKC 蛋白的表达率明显高于癌旁组织，提示这些蛋白与食管癌的发生和发展有关。这些蛋白之间的相关性也表明它们在食管癌的发展过程中可能发挥协同作用。
