Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR China; Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, Canada.
Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, Canada.
Biosens Bioelectron. 2018 Jan 15;99:259-267. doi: 10.1016/j.bios.2017.07.064. Epub 2017 Jul 27.
Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS-based cell functional assay for testing cell migration (the M). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M. In addition to research applications, we demonstrated the effective use of the M for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.
基于微流控装置和智能手机集成的移动感应，即 MS 技术，近年来已经实现了许多应用，并继续激发研究界和工业界越来越多的兴趣。特别是，人们设想 MS 技术可以用于各种细胞功能测定，以实现基础研究和临床应用。为此，在本文中，我们描述了一种基于 MS 的细胞功能测定方法的开发，用于测试细胞迁移（M）。该系统构建为一个集成测试套件，包括微流控芯片、基于智能手机的成像平台、用于图像捕获和数据分析的手机应用程序，以及一组用于进行细胞迁移测定的试剂和附件。我们证明 M 可以有效地测量纯化的中性粒细胞和癌细胞的趋化性。此外，M 可以通过 RBC 裂解从一滴全血中测试中性粒细胞趋化性。还使用 M 测试了趋化剂剂量和梯度分布对中性粒细胞趋化性的影响。除了研究应用之外，我们还证明了 M 可以在医院现场测试和测试慢性阻塞性肺疾病患者的临床样本方面的有效应用。因此，这种开发的 M 为细胞迁移相关研究和潜在的医学诊断应用提供了一个简单集成的实验平台。
