Jonitz-Heincke Anika, Tillmann Jenny, Klinder Annett, Krueger Simone, Kretzer Jan Philippe, Høl Paul Johan, Paulus Alexander C, Bader Rainer
Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medical Center Rostock, 18057 Rostock, Germany.
Clinic for Orthopaedics and Trauma Surgery, Heidelberg University Hospital, 69118 Heidelberg, Germany.
Materials (Basel). 2017 Jul 3;10(7):734. doi: 10.3390/ma10070734.
Osteolysis in the periprosthetic tissue can be caused by metallic wear particles and ions that can originate from implant surface corrosion. These products influence cellular behavior and stimulate the expression of proinflammatory cytokines. The purpose of this study was to evaluate the impact of CoCr29Mo6 ions on cell survival, differentiation, and cytokine expression in human osteoblasts and peripheral blood mononuclear cells (PBMCs). Thus, we exposed cells with a mixture of 200 µg/L ion solution and determined cell viability and apoptosis/necrosis. Gene expression analyses of osteoblastic and osteoclastic differentiation markers as well as pro-osteolytic mediators (, , , , , ) were performed. These markers were also investigated in mixed cultures of adherent and non-adherent PBMCs as well as in co-cultures of human osteoblasts and PBMCs. The ion solution induced necrosis in osteoblasts and PBMCs in single cultures. All examined mediators were highly expressed in the co-culture of osteoblasts and PBMCs whereas in the single cell cultures only , , and were found to be stimulated. While the applied concentration of the CoCr29Mo6 ion solutions had only marginal effects on human osteoblasts and PBMCs alone, the co-culture may provide a comprehensive model to study osteolytic processes in response to Co and Cr ions.
假体周围组织中的骨溶解可能由金属磨损颗粒和离子引起，这些颗粒和离子可能源于植入物表面腐蚀。这些产物影响细胞行为并刺激促炎细胞因子的表达。本研究的目的是评估CoCr29Mo6离子对人成骨细胞和外周血单核细胞（PBMC）的细胞存活、分化及细胞因子表达的影响。因此，我们用200 µg/L离子溶液混合物处理细胞，并测定细胞活力和凋亡/坏死情况。进行了成骨细胞和成骨细胞分化标志物以及促骨溶解介质（，，，，，）的基因表达分析。这些标志物也在贴壁和非贴壁PBMC的混合培养物以及人成骨细胞和PBMC的共培养物中进行了研究。离子溶液在单一培养物中诱导成骨细胞和PBMC坏死。所有检测的介质在成骨细胞和PBMC的共培养物中高表达，而在单细胞培养物中仅发现，，和受到刺激。虽然CoCr29Mo6离子溶液的应用浓度单独对人成骨细胞和PBMC仅有轻微影响，但共培养可能提供一个综合模型来研究对Co和Cr离子的骨溶解过程。
