通过熔体静电纺丝制备的用于骨组织工程的聚（ε-己内酯）支架
Poly(ε-caprolactone) Scaffolds Fabricated by Melt Electrospinning for Bone Tissue Engineering.
作者信息
Zaiss Sascha, Brown Toby D, Reichert Johannes C, Berner Arne
机构信息
Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland 4001, Australia.
Department of Trauma Surgery, University Hospital Regensburg, Regensburg 93055, Germany.
出版信息
Materials (Basel). 2016 Mar 25;9(4):232. doi: 10.3390/ma9040232.
Melt electrospinning is a promising approach to manufacture biocompatible scaffolds for tissue engineering. In this study, melt electrospinning of poly(ε-caprolactone) onto structured, metallic collectors resulted in scaffolds with an average pore size of 250-300 μm and an average fibre diameter of 15 μm. Scaffolds were seeded with ovine osteoblasts . Cell proliferation and deposition of mineralised extracellular matrix was assessed using PicoGreen (Thermo Fisher Scientific, Scoresby, Australia) and WAKO HR II (WAKO, Osaka, Japan) calcium assays. Biocompatibility, cell infiltration and the growth pattern of osteoblasts on scaffolds was investigated using confocal microscopy and scanning electron microscopy. Osteoblasts proliferated on the scaffolds over an entire 40-day culture period, with excellent survival rates and deposited mineralized extracellular matrix. In general, the 3D environment of the structured melt electrospun scaffold was favourable for osteoblast cultures.
熔体静电纺丝是一种用于制造组织工程生物相容性支架的很有前景的方法。在本研究中，将聚（ε-己内酯）熔体静电纺丝到结构化金属收集器上，得到平均孔径为250-300μm且平均纤维直径为15μm的支架。将羊成骨细胞接种到支架上。使用PicoGreen（赛默飞世尔科技，澳大利亚斯科斯比）和WAKO HR II（和光纯药，日本大阪）钙测定法评估细胞增殖和矿化细胞外基质的沉积。使用共聚焦显微镜和扫描电子显微镜研究支架上成骨细胞的生物相容性、细胞浸润和生长模式。在整个40天的培养期内，成骨细胞在支架上增殖，具有优异的存活率并沉积了矿化细胞外基质。一般来说，结构化熔体静电纺丝支架的三维环境有利于成骨细胞培养。
Zotero 插件