Paladini Federica, Di Franco Cinzia, Panico Angelica, Scamarcio Gaetano, Sannino Alessandro, Pollini Mauro
Department of Engineering for Innovation, University of Salento, Via per Monteroni, Lecce 73100, Italy.
CNR-IFN U.O.S. Bari, Via Amendola 173, Bari 70126, Italy.
Materials (Basel). 2016 May 25;9(6):411. doi: 10.3390/ma9060411.
Multidrug-resistant organisms are increasingly implicated in acute and chronic wound infections, thus compromising the chance of therapeutic options. The resistance to conventional antibiotics demonstrated by some bacterial strains has encouraged new approaches for the prevention of infections in wounds and burns, among them the use of silver compounds and nanocrystalline silver. Recently, silver wound dressings have become widely accepted in wound healing centers and are commercially available. In this work, novel antibacterial wound dressings have been developed through a silver deposition technology based on the photochemical synthesis of silver nanoparticles. The devices obtained are completely natural and the silver coatings are characterized by an excellent adhesion without the use of any binder. The silver-treated cotton gauzes were characterized through scanning electron microscopy (SEM) and thermo-gravimetric analysis (TGA) in order to verify the distribution and the dimension of the silver particles on the cotton fibers. The effectiveness of the silver-treated gauzes in reducing the bacterial growth and biofilm proliferation has been demonstrated through agar diffusion tests, bacterial enumeration test, biofilm quantification tests, fluorescence and SEM microscopy. Moreover, potential cytotoxicity of the silver coating was evaluated through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide colorimetric assay (MTT) and the extract method on fibroblasts and keratinocytes. Inductively coupled plasma mass spectrometry (ICP-MS) was performed in order to determine the silver release in different media and to relate the results to the biological characterization. All the results obtained were compared with plain gauzes as a negative control, as well as gauzes treated with a higher silver percentage as a positive control.
多重耐药菌越来越多地与急慢性伤口感染相关，从而降低了治疗选择的机会。一些细菌菌株对传统抗生素的耐药性促使人们采用新方法来预防伤口和烧伤感染，其中包括使用银化合物和纳米晶银。最近，银伤口敷料在伤口愈合中心已被广泛接受且有商业产品可供使用。在这项工作中，通过基于银纳米颗粒光化学合成的银沉积技术开发了新型抗菌伤口敷料。所获得的器件完全天然，银涂层的特点是无需使用任何粘合剂即可实现优异的附着力。通过扫描电子显微镜（SEM）和热重分析（TGA）对经银处理的棉纱布进行表征，以验证银颗粒在棉纤维上的分布和尺寸。通过琼脂扩散试验、细菌计数试验、生物膜定量试验、荧光和SEM显微镜观察，证明了经银处理的纱布在减少细菌生长和生物膜增殖方面的有效性。此外，通过3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐比色法（MTT）以及对成纤维细胞和角质形成细胞的提取物法评估了银涂层的潜在细胞毒性。进行电感耦合等离子体质谱（ICP-MS）以确定不同介质中的银释放量，并将结果与生物学表征相关联。将所有获得的结果与作为阴性对照的普通纱布以及作为阳性对照的用更高银含量处理的纱布进行比较。
