Yoshida Kentaro, Suwa Keisuke, Anzai Jun-Ichi
School of Pharmaceutical Sciences, Ohu University, 31-1 Misumido, Tomita-machi, Koriyama, Fukushima 963-8611, Japan.
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Materials (Basel). 2016 May 28;9(6):425. doi: 10.3390/ma9060425.
Layer-by-layer films composed of polysaccharides and poly(amidoamine) dendrimer bearing phenylboronic acid (PBA-PAMAM) were prepared to study the deposition behavior of the films and their stability in buffer solutions and in sugar solutions. Alginic acid (AGA) and carboxymethylcellulose (CMC) were employed as counter-polymers in constructing LbL films. AGA/PBA-PAMAM films were successfully prepared at pH 6.0-9.0, whereas the preparation of CMC/PBA-PAMAM film was unsuccessful at pH 8.0 and 9.0. The results show that the LbL films formed mainly through electrostatic affinity between PBA-PAMAM and polysaccharides, while, for AGA/PBA-PAMAM films, the participation of boronate ester bonds in the films was suggested. AGA/PBA-PAMAM films were stable in the solutions of pH 6.0-9.0. In contrast, CMC/PBA-PAMAM films decomposed at pH 7.5-9.0. The AGA/PBA-PAMAM films decomposed in response to 5-30 mM fructose at pH 7.5, while the films were stable in glucose solutions. Thus, AGA is useful as a counter-polymer for constructing PBA-PAMAM films that are stable at physiological pH and decompose in response to fructose.
制备了由多糖和带有苯硼酸的聚（酰胺胺）树枝状大分子（PBA-PAMAM）组成的逐层膜，以研究膜的沉积行为及其在缓冲溶液和糖溶液中的稳定性。在构建逐层膜时，使用了海藻酸（AGA）和羧甲基纤维素（CMC）作为反聚合物。AGA/PBA-PAMAM膜在pH 6.0-9.0时成功制备，而CMC/PBA-PAMAM膜在pH 8.0和9.0时制备不成功。结果表明，逐层膜主要通过PBA-PAMAM与多糖之间的静电亲和力形成，而对于AGA/PBA-PAMAM膜，推测膜中存在硼酸酯键的参与。AGA/PBA-PAMAM膜在pH 6.0-9.0的溶液中稳定。相比之下，CMC/PBA-PAMAM膜在pH 7.5-9.0时分解。AGA/PBA-PAMAM膜在pH 7.5时对5-30 mM果糖有响应而分解，而该膜在葡萄糖溶液中稳定。因此，AGA作为构建在生理pH下稳定且对果糖有响应而分解的PBA-PAMAM膜的反聚合物是有用的。
