Allitto B A, Karrer K M
Nucleic Acids Res. 1986 Oct 24;14(20):8007-25. doi: 10.1093/nar/14.20.8007.
A small family of DNA sequences is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which constitute the macronuclear family. Genomic Southern hybridization experiments indicate there are five members of the family in micronuclear DNA. All of the family members are present in whole genome homozygotes and are therefore nonallelic. The three macronuclear sequences are all present in clonal cell lines and are reproducibly generated in every developing macronucleus. The rearrangement event begins 14 hours after conjugation is initiated and is nearly completed by 16 hours.
在四膜虫体细胞细胞核发育过程中，一小部分DNA序列发生了重排。该家族由266 bp的高度保守序列定义，限制性图谱分析、杂交和序列分析表明，一个克隆的微核片段以及构成大核家族的三个序列共享该保守序列。基因组Southern杂交实验表明，微核DNA中有该家族的五个成员。所有家族成员都存在于全基因组纯合子中，因此是非等位基因。这三个大核序列都存在于克隆细胞系中，并且在每个发育中的大核中都能重复产生。重排事件在接合开始后14小时开始，到16小时时几乎完成。
