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1
A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.一种利用聚合酶链反应绘制嗜热四膜虫种系序列图谱的方法。
Genetics. 1994 May;137(1):95-106. doi: 10.1093/genetics/137.1.95.
2
Germ line-specific DNA sequences are present on all five micronuclear chromosomes in Tetrahymena thermophila.种系特异性DNA序列存在于嗜热四膜虫的所有五条微核染色体上。
Mol Cell Biol. 1983 Nov;3(11):1909-19. doi: 10.1128/mcb.3.11.1909-1919.1983.
3
Genome-wide characterization of Tetrahymena thermophila chromosome breakage sites. II. Physical and genetic mapping.嗜热四膜虫染色体断裂位点的全基因组特征分析。II. 物理和遗传图谱分析。
Genetics. 2005 Aug;170(4):1623-31. doi: 10.1534/genetics.104.031435. Epub 2005 Jun 14.
4
Mapping the germ-line and somatic genomes of a ciliated protozoan, Tetrahymena thermophila.绘制纤毛原生动物嗜热栖热四膜虫的种系基因组和体细胞基因组图谱。
Genome Res. 1998 Feb;8(2):91-9. doi: 10.1101/gr.8.2.91.
5
Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila.嗜热四膜虫随机扩增多态性DNA的鉴定、定位及连锁分析
Genetics. 1996 Jun;143(2):811-21. doi: 10.1093/genetics/143.2.811.
6
Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila.与嗜热四膜虫交配型位点紧密连锁的随机扩增多态性DNA的遗传图谱。
Genetics. 1995 Dec;141(4):1315-25. doi: 10.1093/genetics/141.4.1315.
7
An unusual sequence arrangement in the telomeres of the germ-line micronucleus in Tetrahymena thermophila.嗜热四膜虫生殖系微核端粒中一种不寻常的序列排列。
Genes Dev. 1995 Jan 1;9(1):59-71. doi: 10.1101/gad.9.1.59.
8
A novel family of mobile genetic elements is limited to the germline genome in Tetrahymena thermophila.一个新的可移动遗传元件家族仅限于嗜热四膜虫的生殖系基因组。
Nucleic Acids Res. 2002 Jun 1;30(11):2524-37. doi: 10.1093/nar/30.11.2524.
9
Detection of circular excised DNA deletion elements in Tetrahymena thermophila during development.嗜热四膜虫发育过程中环状切除型DNA缺失元件的检测
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A small family of elements with long inverted repeats is located near sites of developmentally regulated DNA rearrangement in Tetrahymena thermophila.在嗜热四膜虫中，一小类具有长反向重复序列的元件位于发育调控的DNA重排位点附近。
Mol Cell Biol. 1994 Sep;14(9):5939-49. doi: 10.1128/mcb.14.9.5939-5949.1994.

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The 'Janus A' gene encodes a polo-kinase whose loss creates a dorsal/ventral intracellular homeosis in the ciliate, .“贾纳斯A”基因编码一种波罗蛋白激酶，其缺失会在纤毛虫中产生背/腹细胞内同源异型现象。
bioRxiv. 2024 Dec 20:2024.12.19.629484. doi: 10.1101/2024.12.19.629484.
2
Micronucleus-specific histone H1 is required for micronuclear chromosome integrity in Tetrahymena thermophila.微小核特异性组蛋白H1是嗜热四膜虫微小核染色体完整性所必需的。
PLoS One. 2017 Nov 2;12(11):e0187475. doi: 10.1371/journal.pone.0187475. eCollection 2017.
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Structure of the germline genome of and relationship to the massively rearranged somatic genome.种系基因组的结构及其与大量重排的体细胞基因组的关系。
Elife. 2016 Nov 28;5:e19090. doi: 10.7554/eLife.19090.
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The conjugation-specific Die5 protein is required for development of the somatic nucleus in both Paramecium and Tetrahymena.在草履虫和四膜虫中，特异性结合的Die5蛋白是体细胞核发育所必需的。
Eukaryot Cell. 2010 Jul;9(7):1087-99. doi: 10.1128/EC.00379-09. Epub 2010 May 21.
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The highly conserved family of Tetrahymena thermophila chromosome breakage elements contains an invariant 10-base-pair core.嗜热四膜虫染色体断裂元件的高度保守家族包含一个不变的10个碱基对的核心。
Eukaryot Cell. 2006 Apr;5(4):771-80. doi: 10.1128/EC.5.4.771-780.2006.
6
Genome-wide characterization of tetrahymena thermophila chromosome breakage sites. I. Cloning and identification of functional sites.嗜热四膜虫染色体断裂位点的全基因组特征分析。I. 功能位点的克隆与鉴定。
Genetics. 2005 Aug;170(4):1611-21. doi: 10.1534/genetics.104.031401. Epub 2005 Jun 14.
7
Role of micronucleus-limited DNA in programmed deletion of mse2.9 during macronuclear development of Tetrahymena thermophila.微核限制DNA在嗜热四膜虫大核发育过程中mse2.9程序性缺失中的作用。
Eukaryot Cell. 2004 Apr;3(2):288-301. doi: 10.1128/EC.3.2.288-301.2004.
8
Nongenic, bidirectional transcription precedes and may promote developmental DNA deletion in Tetrahymena thermophila.非基因的双向转录先于嗜热四膜虫的发育性DNA缺失并可能促进其发生。
Genes Dev. 2001 May 15;15(10):1287-98. doi: 10.1101/gad.884601.
9
Flanking regulatory sequences of the Tetrahymena R deletion element determine the boundaries of DNA rearrangement.嗜热四膜虫R缺失元件的侧翼调控序列决定了DNA重排的边界。
Mol Cell Biol. 1999 Aug;19(8):5631-41. doi: 10.1128/MCB.19.8.5631.
10
Mapping three classical isozyme loci in tetrahymena: meiotic linkage of EstA to the ChxA linkage group.在四膜虫中定位三个经典同工酶基因座：酯酶A与ChxA连锁群的减数分裂连锁
Genetics. 1996 Dec;144(4):1489-96. doi: 10.1093/genetics/144.4.1489.

本文引用的文献

1
Nullisomic tetrahymena: eliminating germinal chromosomes.单体四膜虫：消除生殖染色体。
Science. 1981 Jul 31;213(4507):549-51. doi: 10.1126/science.213.4507.549.
2
Linkage and Genetic Map Length in TETRAHYMENA THERMOPHILA.四膜虫中的连锁与遗传图谱长度。
Genetics. 1977 Nov;87(3):421-39. doi: 10.1093/genetics/87.3.421.
3
Regulatory Serotype Mutations in TETRAHYMENA PYRIFORMIS, Syngen 1.四膜虫，同型共生 1 中的调节血清型突变。
Genetics. 1973 May;74(1):81-106. doi: 10.1093/genetics/74.1.81.
4
Nullisomic Tetrahymena. II. a Set of Nullisomics Define the Germinal Chromosomes.单体型四膜虫。二。一组单体型定义了生殖染色体。
Genetics. 1983 Jun;104(2):257-70. doi: 10.1093/genetics/104.2.257.
5
Genome organization and reorganization in Tetrahymena.四膜虫的基因组组织与重组
Annu Rev Genet. 1980;14:203-39. doi: 10.1146/annurev.ge.14.120180.001223.
6
Chromosome-designated mutation selection in Tetrahymena thermophila.嗜热四膜虫中染色体指定的突变选择
Genetics. 1984 Mar;106(3):387-401. doi: 10.1093/genetics/106.3.387.
7
The 5S ribosomal RNA gene clusters in Tetrahymena thermophila: strain differences, chromosomal localization, and loss during micronuclear ageing.嗜热四膜虫中的5S核糖体RNA基因簇：菌株差异、染色体定位及小核衰老过程中的丢失
Mol Gen Genet. 1984;197(2):244-53. doi: 10.1007/BF00330970.
8
DNA elimination in Tetrahymena: a developmental process involving extensive breakage and rejoining of DNA at defined sites.四膜虫中的DNA消除：一个涉及在特定位点大量DNA断裂和重新连接的发育过程。
Cell. 1984 Feb;36(2):433-40. doi: 10.1016/0092-8674(84)90236-8.
9
Ribosomal RNA gene amplification in Tetrahymena may be associated with chromosome breakage and DNA elimination.四膜虫中的核糖体RNA基因扩增可能与染色体断裂和DNA消除有关。
Cell. 1981 Jun;24(3):765-74. doi: 10.1016/0092-8674(81)90102-1.
10
Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development.四膜虫核糖体DNA自由末端的串联重复六核苷酸在发育过程中由单拷贝产生。
Cell. 1982 Nov;31(1):177-82. doi: 10.1016/0092-8674(82)90417-2.

一种利用聚合酶链反应绘制嗜热四膜虫种系序列图谱的方法。

A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.

作者信息

Cassidy-Hanley D, Yao M C, Bruns P J

机构信息

Section of Genetics and Development, Cornell University, Ithaca, New York 14853.

出版信息

Genetics. 1994 May;137(1):95-106. doi: 10.1093/genetics/137.1.95.

DOI:10.1093/genetics/137.1.95
PMID:8056326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1205958/
Abstract

A method for mapping DNA sequences to specific germinal chromosomes in the ciliated protozoan Tetrahymena thermophila has been developed. This mapping technique (PCR mapping) utilizes the polymerase chain reaction and template DNA derived from nullisomic strains to directly assign micronuclear DNA sequences to specific micronuclear chromosomes. Using this technique, a number of unique sequences and short repetitive sequences flanked by unique sequences have been mapped to four of the five germinal chromosomes.

摘要

已经开发出一种将DNA序列定位到嗜热四膜虫纤毛原生动物特定生殖染色体上的方法。这种定位技术（PCR定位）利用聚合酶链反应和来自缺体菌株的模板DNA，将微核DNA序列直接定位到特定的微核染色体上。利用该技术，一些独特序列和两侧为独特序列的短重复序列已被定位到五条生殖染色体中的四条上。