一种利用聚合酶链反应绘制嗜热四膜虫种系序列图谱的方法。
A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.
作者信息
Cassidy-Hanley D, Yao M C, Bruns P J
机构信息
Section of Genetics and Development, Cornell University, Ithaca, New York 14853.
出版信息
Genetics. 1994 May;137(1):95-106. doi: 10.1093/genetics/137.1.95.
Abstract
A method for mapping DNA sequences to specific germinal chromosomes in the ciliated protozoan Tetrahymena thermophila has been developed. This mapping technique (PCR mapping) utilizes the polymerase chain reaction and template DNA derived from nullisomic strains to directly assign micronuclear DNA sequences to specific micronuclear chromosomes. Using this technique, a number of unique sequences and short repetitive sequences flanked by unique sequences have been mapped to four of the five germinal chromosomes.
摘要
已经开发出一种将DNA序列定位到嗜热四膜虫纤毛原生动物特定生殖染色体上的方法。这种定位技术（PCR定位）利用聚合酶链反应和来自缺体菌株的模板DNA，将微核DNA序列直接定位到特定的微核染色体上。利用该技术，一些独特序列和两侧为独特序列的短重复序列已被定位到五条生殖染色体中的四条上。
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DNA elimination in Tetrahymena: a developmental process involving extensive breakage and rejoining of DNA at defined sites.四膜虫中的DNA消除：一个涉及在特定位点大量DNA断裂和重新连接的发育过程。
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Ribosomal RNA gene amplification in Tetrahymena may be associated with chromosome breakage and DNA elimination.四膜虫中的核糖体RNA基因扩增可能与染色体断裂和DNA消除有关。
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Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development.四膜虫核糖体DNA自由末端的串联重复六核苷酸在发育过程中由单拷贝产生。
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