André Carolina Bosso, Rosalen Pedro Luiz, Galvão Lívia Câmara de Carvalho, Fronza Bruna Marin, Ambrosano Glaucia Maria Bovi, Ferracane Jack Liborio, Giannini Marcelo
Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil.
Department of Physiological Sciences, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil.
Dent Mater. 2017 Oct;33(10):1084-1092. doi: 10.1016/j.dental.2017.07.006. Epub 2017 Jul 31.
The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin.
Apigenin (1mM) and tt-Farnesol (5mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology.
Addition of Apigenin and Apigenin+tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin+tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions.
Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance.
本体外研究旨在分析将两种防龋剂加入牙科黏结剂后，对变形链球菌毒力降低及对牙本质黏附的影响。
将芹菜素（1mM）和金合欢醇（5mM）分别单独及联合添加到一种自酸蚀黏结剂（CS3 - 可乐丽S3 Bond Plus）和一种全酸蚀黏结剂（OPT - OptiBond S）中。变形链球菌生物膜在涂有黏结剂的羟基磷灰石圆盘上生长115小时，对细菌活力、干重、碱溶性、水溶性、细胞内多糖和蛋白质进行定量分析。进行黏结强度和牙本质 - 黏结剂界面分析，以研究添加防龋剂对物理性能的影响，并确定混合层形态的变化。
在CS3中添加芹菜素和芹菜素 + 金合欢醇，以及在OPT中添加芹菜素或金合欢醇，均可降低变形链球菌生物膜的干重。在CS3中添加芹菜素和在OPT中添加金合欢醇可使不溶性多糖减少。在CS3中添加芹菜素和芹菜素 + 金合欢醇可使细胞内多糖减少。添加防龋剂后，牙本质黏结强度、树脂 - 牙本质界面形态、蛋白质总量和可溶性多糖均未观察到变化。
牙修复体周围致龋性较低的生物膜可减少继发龋的形成；此外，在不一定杀死微生物的情况下降低变形链球菌的毒力，更不容易诱导抗菌耐药性。
