将芹菜素和 tt-法呢醇掺入牙科复合材料中以调节变形链球菌的毒力。
Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence.
机构信息
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Department of Biosciences, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil; Biological Sciences Graduate Program, Federal University of Alfenas, Alfenas, MG, Brazil.
出版信息
Dent Mater. 2021 Apr;37(4):e201-e212. doi: 10.1016/j.dental.2020.12.005. Epub 2021 Jan 6.
OBJECTIVES
The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations.
METHODS
Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed.
RESULTS
The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites.
SIGNIFICANCE
Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
目的
本体外研究旨在将两种抗龋剂（芹黄素和 tt-法呢醇）掺入树脂复合材料和树脂水门汀中，以降低牙修复体周围变形链球菌的毒力。
方法
单独添加芹黄素（Api，5mM）和 tt-法呢醇（Far，5mM），联合添加，以及与氟（F）联合添加。将变形链球菌生物膜种植在复合材料圆盘上，定量测量干燥重量、细菌活力以及多糖（碱溶性、细胞内和水溶性）。在水储存 3 年后，获得变形链球菌生物膜的 CLSM 图像。还分析了添加物对物理化学性质和复合材料比色参数的影响。
结果
添加物不影响细菌活力。单独的 Api 以及与 Far 联合或与 Far 和 F 联合添加降低了细菌干燥重量、碱溶性和细胞内多糖。三年后，含有添加物的复合材料在生物膜的顶部呈现出更大的 EPS 基质。转化率有统计学差异；然而，最大聚合速率和固化动力学不受添加物的影响。水溶性多糖、弯曲强度和弹性模量没有差异。Api 增加了复合材料的黄度。
意义
单独或联合使用 Api 降低了变形链球菌的毒力表达，而不会影响复合材料的物理化学性质。
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