MARTX Toxin in the Zoonotic Serovar of Triggers an Early Cytokine Storm in Mice.

biotype 2-serovar E is a zoonotic clonal complex that can cause death by sepsis in humans and fish. Unlike other biotypes, Bt2 produces a unique type of MARTX (Multifunctional-Autoprocessive-Repeats-in-Toxin; RtxA1), which is encoded by a gene duplicated in the pVvBt2 plasmid and chromosome II. In this work, we analyzed the activity of this toxin and its role in human sepsis by performing , and assays. First, we demonstrated that the ACD domain, present exclusively in this toxin variant, effectively has an actin-cross-linking activity. Second, we determined that the whole toxin caused death of human endotheliocytes and monocytes by lysis and apoptosis, respectively. Finally, we tested the hypothesis that RtxA1 contributes to human death caused by this zoonotic serovar by triggering an early cytokine storm in blood. To this end, we used a Bt2-SerE strain (R99) together with its deficient mutant, and a Bt1 strain (YJ016) producing RtxA1 (the most studied MARTX) together with its deficient mutant, as controls. Our results showed that RtxA1 was essential for virulence, as R99ΔΔ was completely avirulent in our murine model of infection, and that R99, but not strain YJ016, induced an early, strong and dysregulated immune response involving the up-regulation of a high number of genes. This dysregulated immune response was directly linked to RtxA1. Based on these results and those obtained (human blood), we propose a model of infection for the zoonotic serovar of , in which RtxA1 would act as a sepsis-inducing toxin.