Murciano Celia, Hor Lien-I, Amaro Carmen
Estructura de Investigación Interdisciplinar en Biotecnología y Medicina (ERI BIOTECMED). Department of Microbiology & Ecology, University of Valencia, 46100 Burjassot, Valencia, Spain.
Future Microbiol. 2015;10(4):471-87. doi: 10.2217/fmb.14.136.
To demonstrate that Vibrio vulnificus, a sepsis-related aquatic pathogen, can provoke a strong pro-inflammatory reaction in blood-associated target cells.
MATERIALS & METHODS: We selected two strains of the two main phylogenetic lineages, two human cell lines, monocytes and vascular endothelial cells and designed an in vitro infection model simulating early septicemia.
Both strains caused a strong cell-specific pro-inflammatory response and produced a high degree of cell damage that ended with death by lysis (endothelial cells) or apoptosis/lysis (monocytes). The interaction with endothelial cells was stronger than expected and significantly different for both lineages.
The early interaction with endothelial cells could have a direct role in sepsis and could explain, at least partially, the differences in pathogenicity between both lineages.
证明与败血症相关的水生病原菌创伤弧菌可在血液相关靶细胞中引发强烈的促炎反应。
我们选择了两个主要系统发育谱系的两株菌株、两种人类细胞系、单核细胞和血管内皮细胞，并设计了一个模拟早期败血症的体外感染模型。
两株菌株均引起强烈的细胞特异性促炎反应，并造成高度的细胞损伤，最终以内皮细胞溶解死亡或单核细胞凋亡/溶解死亡告终。与内皮细胞的相互作用比预期更强，且两个谱系之间存在显著差异。
与内皮细胞的早期相互作用可能在败血症中起直接作用，并且至少可以部分解释两个谱系之间致病性的差异。
