Faculty of Environment and Resource Studies, Mahidol University, Phutthamonthon Sai 4 Rd., Salaya, Phutthamonthon, Nakhon Pathom, 73170, Thailand.
Department of Plant Science, Faculty of Science, Mahidol University, Rama VI Rd., Phayathai, Ratchathewi, Bangkok, 10400, Thailand.
Environ Sci Pollut Res Int. 2017 Sep;24(27):21821-21828. doi: 10.1007/s11356-017-9814-7. Epub 2017 Aug 3.
This study investigated the short-term impacts of an oil spill on the leaf anatomical structures of Terminalia catappa L. from crude oil leakage in Rayong province, Thailand, in 2013. Approximately 3 weeks after the oil spill, leaves of T. catappa were collected along the coastline of Rayong from one affected site, five adjacent sites, and a control site. Slides of the leaf epidermis were prepared by the peeling method, while leaf and petiole transverse sections were prepared by paraffin embedding. Cell walls of adaxial epidermal cell on leaves in the affected site were straight instead of the jigsaw shape found in leaves from the adjacent and control sites. In addition, the stomatal index of the abaxial leaf surface was significantly lower in the affected site. Leaf and petiole transverse sections collected from the affected site showed increased cuticle thickness, epidermal cell diameter on both sides, and palisade mesophyll thickness; in contrast, vessel diameter and spongy mesophyll thickness were reduced. These significant changes in the leaf anatomy of T. catappa correspond with previous research and demonstrate the negative effects of oil spill pollution on plants. The anatomical changes of T. catappa in response to crude oil pollution are discussed as a possible indicator of pollution and may be used in monitoring crude oil pollution.
本研究调查了 2013 年泰国罗勇府原油泄漏事件对桐花树叶片解剖结构的短期影响。溢油事件发生约 3 周后，从罗勇府的一个受影响地点、五个相邻地点和一个对照地点采集了桐花树的叶片。通过剥皮法制备叶片表皮的载玻片，通过石蜡包埋制备叶片和叶柄的横切片。受影响地点叶片的近轴表皮细胞细胞壁是直的，而相邻和对照地点叶片的近轴表皮细胞细胞壁则呈拼图状。此外，受影响地点叶片下表面的气孔指数显著降低。从受影响地点采集的叶片和叶柄横切片显示，角质层厚度、两侧表皮细胞直径和栅栏组织厚度增加；相反，导管直径和海绵组织厚度减少。桐花树叶片解剖结构的这些显著变化与之前的研究一致，表明溢油污染对植物有负面影响。讨论了桐花树对原油污染的解剖学变化作为污染的可能指标，并可用于监测原油污染。
