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衍射蛋白质光栅作为高通量无标记免疫传感的光活性传感器。

Diffractive Protein Gratings as Optically Active Transducers for High-Throughput Label-free Immunosensing.

机构信息

Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València , 46022 Valencia, Spain.

Departmento de Quı́mica, Universitat Politècnica de València , 46022 Valencia, Spain.

出版信息

Anal Chem. 2017 Sep 5;89(17):9002-9008. doi: 10.1021/acs.analchem.7b01649. Epub 2017 Aug 24.

Abstract

A novel label-free biosensing approach based on bioreceptor networks patterned as diffractive gratings (biogratings) has been developed. Nanogrooved structures were used as optically active scaffolds for producing arrays of functional BSA biogratings on low energy surfaces by a water-assisted variant of microcontact printing. An analytical scanner, comprising a LightScribe compact disk drive, was developed to measure the diffraction patterns of these biogratings, thus allowing biointeractions to be quantitatively sensed in a multiplex and label-free fashion by means of diffraction efficiency changes. The approach was demonstrated by immunoassaying IgGs, reaching well-correlated responses with quantification and detection limits of 1.3 and 5.2 nM, respectively. These results provide appealing insights into cost-effective, portable, and scalable alternatives for designing new analytical technologies based on diffractive gratings of bioreceptors.

摘要

一种基于生物受体网络的新型无标记生物传感方法已经被开发出来,该方法采用了作为衍射光栅(生物光栅)图案化的生物受体网络。纳米槽结构被用作光活性支架,通过水辅助的微接触印刷变体在低能量表面上产生功能化 BSA 生物光栅阵列。开发了一种分析扫描仪,包括一个 LightScribe 光盘驱动器,用于测量这些生物光栅的衍射图案,从而可以通过衍射效率变化以多路复用和无标记的方式定量感应生物相互作用。该方法通过免疫测定 IgG 进行了验证,达到了良好相关的响应,定量和检测限分别为 1.3 和 5.2 nM。这些结果为基于生物受体的衍射光栅设计新的分析技术提供了有吸引力的经济实惠、便携和可扩展的替代方案。

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