Department of Chemistry, ‡Environmental Toxicology Program, §Department of Biochemistry and Molecular Biology, University of California-Riverside , Riverside, California 92521, United States.
J Am Chem Soc. 2017 Aug 16;139(32):10964-10967. doi: 10.1021/jacs.7b05002. Epub 2017 Aug 8.
Arrayed deep cavitands can be coupled to a fluorescence-based supramolecular tandem assay that allows site-selective in situ monitoring of post-translational modifications catalyzed by the lysine methyltransferase PRDM9 or the lysine demethylase JMJD2E. An arrayed sensor system containing only three cavitand components can detect the specific substrates of enzyme modification, in the presence of other histone peptides in the enzyme assay, enabling investigation of cross-reactivity over multiple methylation sites and interference from nonsubstrate peptides.
排列密集的深穴穴体可以与基于荧光的超分子串联测定法偶联，从而允许对赖氨酸甲基转移酶 PRDM9 或赖氨酸去甲基酶 JMJD2E 催化的翻译后修饰进行位点选择性的原位监测。一个仅包含三种穴体成分的阵列传感器系统可以在酶测定中存在其他组蛋白肽的情况下检测酶修饰的特定底物，从而能够研究多个甲基化位点的交叉反应性和非底物肽的干扰。
