Xu Wen, Zhang Yiping, Zhou Caijie, Tai Yanni, Zhang Xiaoqing, Liu Jie, Sha Mei, Huang Mingqing, Zhu Yanlin, Peng Jun, Lu Jin-Jian
College of Pharmacy, Fujian Key Laboratory of Chinese Materia Medica, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:314-321. doi: 10.1016/j.jchromb.2017.07.033. Epub 2017 Jul 23.
Pien-Tze-Huang (PZH) is a popular traditional Chinese medicine (TCM) formula in China, but its pharmacokinetics has not been investigated yet. To better study the pharmacokinetic behaviors of PZH, an optimal ultra-performance liquid chromatography with triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for rapid quantification of six compounds (notoginsenoside R1, ginsenosides Re, Rg1, Rb1, Rd, and muscone) in rat plasma after oral administration of PZH. All analytes were extracted by protein precipitation with acetonitrile and separated on a Waters Acquity Cortecs C18 column within 3.9min, and detected by multiple-reaction monitoring in positive ion mode. This proposed method exhibited good linearity (r≥0.9932) with a lower quantification limits of 0.558-1.566ng/mL for all analytes. The intra- and inter-day precisions were within 8.24%, and the accuracy was within -10.05 to 9.87% for each analyte. The extraction recovery for each analyte ranged from 80.02 to 96.12%. This UPLC-MS/MS method was successfully applied to the pharmacokinetic study for PZH in rats.
片仔癀（PZH）是中国一种广受欢迎的中药配方，但尚未对其药代动力学进行研究。为了更好地研究片仔癀的药代动力学行为，开发了一种优化的超高效液相色谱-三重四极杆质谱联用（UPLC-MS/MS）方法，用于快速定量口服片仔癀后大鼠血浆中的六种化合物（三七皂苷R1、人参皂苷Re、Rg1、Rb1、Rd和麝香酮）。所有分析物均通过乙腈蛋白沉淀法提取，并在Waters Acquity Cortecs C18柱上于3.9分钟内分离，采用正离子模式下的多反应监测进行检测。该方法线性良好（r≥0.9932），所有分析物的定量下限为0.558 - 1.566 ng/mL。日内和日间精密度均在8.24%以内，各分析物的准确度在-10.05%至9.87%之间。各分析物的提取回收率在80.02%至96.12%之间。该UPLC-MS/MS方法成功应用于片仔癀在大鼠体内的药代动力学研究。
