Jiang Yue, Li Jie, Ding Meng, Guo Zi-Fan, Yang Hua, Li Hui-Jun, Gao Wen, Li Ping
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China.
Chin Med. 2022 Sep 10;17(1):105. doi: 10.1186/s13020-022-00659-8.
Angong Niuhuang Pill (ANP), a renowned precious traditional Chinese medicine prescription, is extensively utilized for the clinical treatment of stroke, meningitis and encephalorrhagia in China. As a classic resuscitation-inducing aromatic prescription, ANP has been investigated for its pharmacological effects in recent years, while the volatile composition in ANP still lacks comprehensive elucidation.
To better explore the volatile constituents in ANP, a qualitative analysis method was developed based on gas chromatography coupled with mass spectrometry. Furthermore, a validated quantitative method was established to determine 21 main compounds in 8 batches of commercially available ANP samples by gas chromatography-tandem mass spectrometry. The quantitative data were successively subjected to Pearson correlation coefficient analysis. Additionally, the absorbed volatile constituents in rat plasma after single oral administration of ANP have also been characterized.
A total of 93 volatile constituents including 29 sesquiterpenoids, 28 monoterpenoids, 13 fatty acids and their esters, 7 alkanes, 6 ketones, 3 phenols, 3 aldehydes, 2 benzoate esters, and 2 other types, were preliminarily characterized, which primarily originated from Borneolum, Moschus, Curcumae Radix, and Gardeniae Fructus. D-Borneol, isoborneol and muscone were the top three abundant ingredients (> 600 μg/g) in 8 batches of ANP samples. Subsequently, the average Pearson correlation coefficient of the contents of 21 analytes was 0.993, inferring the high batch-to-batch similarity among 8 batches. After oral administration of ANP, D-borneol, isoborneol, muscone and camphor were the main volatile constituents absorbed in the rat plasma.
This research may be helpful for the comprehensive quality control study of ANP, and provide for guarantee the clinical efficacy of ANP.
安宫牛黄丸是著名的名贵中药方剂，在中国广泛用于中风、脑膜炎和脑出血的临床治疗。作为经典的芳香开窍方剂，近年来对安宫牛黄丸的药理作用进行了研究，但其挥发性成分仍缺乏全面的阐释。
为了更好地探究安宫牛黄丸中的挥发性成分，建立了一种基于气相色谱-质谱联用的定性分析方法。此外，还建立了一种经过验证的定量方法，通过气相色谱-串联质谱法测定8批市售安宫牛黄丸样品中的21种主要化合物。对定量数据进行了Pearson相关系数分析。此外，还对大鼠单次口服安宫牛黄丸后血浆中吸收的挥发性成分进行了表征。
初步鉴定出93种挥发性成分，包括29种倍半萜类、28种单萜类、13种脂肪酸及其酯类、7种烷烃、6种酮类、3种酚类、3种醛类、2种苯甲酸酯类和2种其他类型，这些成分主要来源于冰片、麝香、郁金和栀子。右旋龙脑、异龙脑和麝香酮是8批安宫牛黄丸样品中含量最高的三种成分（>600μg/g）。随后，21种分析物含量的平均Pearson相关系数为0.993，表明8批样品之间的批次间相似度较高。口服安宫牛黄丸后，大鼠血浆中吸收的主要挥发性成分为右旋龙脑、异龙脑、麝香酮和樟脑。
本研究可能有助于安宫牛黄丸的全面质量控制研究，并为保证安宫牛黄丸的临床疗效提供依据。
