Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, 88450, Kota Kinabalu, Sabah, Malaysia.
Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Biosciences and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higasi-ku, Fukuoka, 812-8581, Japan.
Microb Ecol. 2018 Feb;75(2):459-467. doi: 10.1007/s00248-017-1043-6. Epub 2017 Aug 4.
Decline in forest productivity due to forest conversion is defining the Bornean landscape. Responses of bacterial communities due to land-use changes are vital and could define our understanding of ecosystem functions. This study reports the changes in bacterial community structure in organic soil (0-5 cm; O-Horizon) and organic-mineral soil (5-15 cm; A-Horizon) across Maliau Basin Conservation Area old growth forest (MBOG), Fragment E logged forest (FELF) located in Kalabakan Forest Reserve to Benta Wawasan oil palm plantation (BWOP) using two-step PCR amplicon analysis of bacteria DNA on Illumina Miseq next generation sequencing. A total of 30 soil samples yielded 893,752-OTU reads at ≥97% similarity from 5,446,512 good quality sequences. Soil from BWOP plantation showed highest unshared OTUs for organic (49.2%) and organic-mineral (50.9%) soil. MBOG soil showed a drop in unshared OTUs between organic (48.6%) and organic-mineral (33.9%). At phylum level, Proteobacteria dominated MBOG but shifted to Actinobacteria in logged and plantation soil. Present findings also indicated that only FELF exhibited change in bacterial communities along the soil depth, moving from the organic to the organic-mineral layer. Both layers of BWOP plantation soils deviated from other forests' soil in β-diversity analysis. To our knowledge, this is the first report on transitions of bacterial community structures with different soil horizons in the tropical rainforest including Borneo, Sabah. Borneo tropical soils form a large reservoir for soil bacteria and future exploration is needed for fully understanding the diversity structure and their bacterial functional properties.
由于森林转换导致的森林生产力下降正在定义婆罗洲的景观。由于土地利用变化而导致的细菌群落的响应至关重要，并可能定义我们对生态系统功能的理解。本研究报告了在马廖盆地保护区原生林（MBOG）、位于卡拉巴干森林保护区的 Fragment E 伐木森林（FELF）和本塔望萨棕榈油种植园（BWOP）的有机土壤（0-5cm；O 层）和有机-矿物土壤（5-15cm；A 层）中细菌群落结构的变化，方法是使用两步 PCR 扩增子分析 Illumina Miseq 下一代测序的细菌 DNA。从 5446512 个高质量序列中，总共 30 个土壤样本产生了 893752 个 OTU 读数，相似度≥97%。BWOP 种植园的土壤在有机（49.2%）和有机-矿物（50.9%）土壤中显示出最高的未共享 OTU。MBOG 土壤的有机（48.6%）和有机-矿物（33.9%）土壤之间的未共享 OTU 下降。在门水平上，变形菌门在 MBOG 中占主导地位，但在伐木和种植园土壤中转变为放线菌门。目前的研究结果还表明，只有 FELF 显示出细菌群落沿土壤深度的变化，从有机层向有机-矿物层移动。BWOP 种植园土壤的两个层在 β 多样性分析中与其他森林土壤不同。据我们所知，这是第一个关于婆罗洲、沙巴热带雨林不同土壤层次的细菌群落结构变化的报告。婆罗洲热带土壤是土壤细菌的一个大储库，需要进一步探索，以充分了解其多样性结构和细菌功能特性。
