Departamento de Ingeniería Química y Tecnología del Medio Ambiente, Universidad de Valladolid, c/Dr. Mergelina s/n, 47011 Valladolid, Spain.
Grupo Procesos Químicos Industriales, Facultad de Ingeniería, Universidad de Antioquia U de A, C/ 70 # 52-21, Medellín, Colombia.
Bioresour Technol. 2017 Nov;244(Pt 1):166-174. doi: 10.1016/j.biortech.2017.07.139. Epub 2017 Jul 27.
Brewer's spent grain (BSG) is a promising feedstock for ABE fermentation. Sulfuric acid pretreatment of BSG at pH 1, 121°C and different solid loadings (5-15% w/w) was investigated. Enzymatic hydrolysis and ABE fermentation by Clostridium beijerinckii DSM 6422 of non-washed and washed pretreated BSG were performed to compare monosaccharide release and butanol production. Pretreatment at 15% w/w BSG resulted in higher availability of sugars in both the enzymatic hydrolysates and pretreatment liquid, and overall yields of 75gbutanol/kg BSG and 95gABE/kg BSG were obtained. When the enzymatic hydrolysate from the washed pretreated BSG was fermented, butanol (6.0±0.5g/L) and ABE (7.4±1.0g/L) concentrations were lower compared with 7.5±0.6g/L butanol and 10.0±0.8g/L ABE from a control. The fermentation of the liquid released in the pretreatment at 15% w/w resulted in a butanol production of 6.6±0.8g/L with a total ABE of 8.6±1.3g/L after overliming.
啤酒糟（BSG）是一种很有前途的ABE 发酵原料。在 pH 值为 1、121°C 及不同固载量（5-15%w/w）条件下，对 BSG 进行硫酸预处理。对未经洗涤和洗涤预处理的 BSG 进行酶水解和 Clostridium beijerinckii DSM 6422 的 ABE 发酵，以比较单糖释放和丁醇生产。在 15%w/w 的 BSG 预处理条件下，酶水解产物和预处理液中糖的利用率更高，最终获得了 75g 丁醇/kg BSG 和 95g ABE/kg BSG 的总产率。当对经洗涤预处理的 BSG 的酶水解产物进行发酵时，与对照相比，丁醇（6.0±0.5g/L）和 ABE（7.4±1.0g/L）浓度较低，分别为 7.5±0.6g/L 丁醇和 10.0±0.8g/L ABE。在 15%w/w 的固载量下进行预处理时释放的液体进行发酵，可得到 6.6±0.8g/L 的丁醇，总 ABE 为 8.6±1.3g/L，经石灰处理后。
