Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Biomed Pharmacother. 2017 Oct;94:481-488. doi: 10.1016/j.biopha.2017.07.133. Epub 2017 Aug 2.
Annona muricata, locally known as soursop has been reported to exhibit antiproliferative activities against various cancer cell lines. In this current study, we have investigated the antitumor promotion of various fractions of Annona muricata leaves (AML); hexane (AMLH), dichloromethane (AMLD) and methanol (AMLM) fraction respectively on 7, 12-dimethylbenz[α]anthracene (DMBA) induced and 12-0-tetradecaboylphorbol-13-acetate (TPA) promoted skin tumorigenesis in mice via morphological assessment, biochemical analysis and histopathological evaluation. The results of the study revealed significant inhibition in tumor incidence, tumor burden and tumor volume in the groups received AMLH and AMLD, respectively, and suppressive effects in group received AMLM compared with carcinogen control group at week 21. Superoxide dismutase, catalase, and lipid peroxidation levels were returned to near normal by administration of AML to DMBA/TPA-induced mice. The above findings were supported by histopathological studies, in which the extensive epidermal hyperplasia in carcinogen control group was restored to normal in AML treated groups. Whilst, annonacin, a major annaonaceous acetogenin was found to be the highest in AMLH and AMLD. From the present study, it can be inferred that AML supressed DMBA/TPA-induced skin tumor and this antitumor-promoting activity may be linked to the antioxidant/free radical-scavenging constituents of the extract and annonacin contained in the extracts.
番荔枝，俗称“赖葡萄”，已被报道具有抗多种癌细胞系增殖的活性。在本研究中，我们分别研究了番荔枝叶的不同馏分（AML），即正己烷（AMLH）、二氯甲烷（AMLD）和甲醇（AMLM）馏分，对 7,12-二甲基苯并[a]蒽（DMBA）诱导和 12-O-十四烷酰佛波醇-13-醋酸酯（TPA）促进的小鼠皮肤肿瘤发生的肿瘤促进作用，通过形态评估、生化分析和组织病理学评价。研究结果表明，AMLH 和 AMLD 组的肿瘤发生率、肿瘤负荷和肿瘤体积均显著抑制，与致癌剂对照组相比，AMLM 组的抑制作用更为显著，在第 21 周。超氧化物歧化酶、过氧化氢酶和脂质过氧化水平通过给予 AML 至 DMBA/TPA 诱导的小鼠而恢复正常。这些发现得到了组织病理学研究的支持，在致癌剂对照组中广泛的表皮过度增生在 AML 处理组中恢复正常。而，番荔枝内酯，一种主要的番荔枝烷类乙酰氧基化合物，在 AMLH 和 AMLD 中含量最高。从本研究可以推断，AML 抑制了 DMBA/TPA 诱导的皮肤肿瘤，这种抗肿瘤促进活性可能与提取物中的抗氧化剂/自由基清除成分和提取物中所含的番荔枝内酯有关。
