Mittal Lakshya, Raman Vishak, Camarillo Ignacio G, Sundararajan Raji
School of Engineering Technology, Purdue University, West Lafayette, IN 47907, USA.
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Biochem Biophys Res Commun. 2017 Sep 30;491(4):1015-1020. doi: 10.1016/j.bbrc.2017.08.002. Epub 2017 Aug 2.
Triple negative breast cancer (TNBC) is difficult to treat due to lack of the three receptors, commonly used for treating breast cancers. Current standard of cure is either ineffective or refractive to many patients. Thus, there is a critical need for alternate, affordable therapies for TNBC cancers. Towards this, electrical pulse-mediated chemotherapy, known as electrochemotherapy is a viable option, because it uses the synergy of electrical pulses and the anticancer properties of chemo drug. Considering the cost and the harsh side effects of various commonly administered chemo drugs, in this study, low cost, yet effective, natural phytochemical curcumin is studied for its anticancer effect on MDA-MB-231, TNBC cells. We applied eight 10 μs, 2500 V/cm or 5000 V/cm pulses with 10 μM concentration of curcumin, and measured cell viability and cytotoxicity. Results indicate that cell survival, as low as 4% was induced by 5000 V/cm pulses, after 72 h, while it was 15% after 24 h. This demonstrates the potential of this treatment for TNBC and the transfer to clinical practice.
三阴性乳腺癌（TNBC）因缺乏常用于治疗乳腺癌的三种受体而难以治疗。目前的标准治疗方法对许多患者无效或产生耐药性。因此，迫切需要针对TNBC癌症的替代且经济实惠的治疗方法。为此，电脉冲介导的化疗，即电化学疗法是一种可行的选择，因为它利用了电脉冲与化疗药物抗癌特性的协同作用。考虑到各种常用化疗药物的成本和严重副作用，在本研究中，对低成本但有效的天然植物化学物质姜黄素对TNBC细胞MDA-MB-231的抗癌作用进行了研究。我们应用了八个10微秒、2500伏/厘米或5000伏/厘米的脉冲以及10微摩尔浓度的姜黄素，并测量了细胞活力和细胞毒性。结果表明，在72小时后，5000伏/厘米的脉冲诱导的细胞存活率低至4%，而在24小时后为15%。这证明了这种治疗方法对TNBC的潜力以及向临床实践的转化。
