Radiotherapy & Chemotherapy Dept. 2, Ningbo NO. 2 Hospital, Zhejiang, China.
Health and Human Service Department, Xiamen Xianyue Hospital, Xiamen, China.
Int J Biol Macromol. 2018 Jan;106:234-240. doi: 10.1016/j.ijbiomac.2017.07.184. Epub 2017 Aug 2.
Aberrant expression of miR-224 is usually found in cancer studies; however, the role of miR-224 has seldom been reported in bladder cancer (BC). We explored miR-224's function and the underlying mechanism in BC. It was found that miR-224 expression was significantly up-regulated in BC tissues and cell lines. Knockdown of miR-224 decreased BC cell growth and invasion both in vitro and in vivo. We identified the SUFU protein as a downstream target of miR-224 by using luciferase and western blot assays. We proposed that miR-224 promoted BC cell growth and invasion via sustaining the activity of Hedgehog pathway, which was negatively regulated by SUFU. Taken together, our study demonstrated that miR-224 may function as an onco-miR in BC and suggested that miR-224 may be a potential therapeutic target for BC patients.
miR-224 的异常表达通常在癌症研究中被发现；然而，miR-224 在膀胱癌（BC）中的作用却很少被报道。我们探索了 miR-224 在 BC 中的功能和潜在机制。研究发现，miR-224 在 BC 组织和细胞系中的表达显著上调。通过体外和体内实验，下调 miR-224 均降低了 BC 细胞的生长和侵袭能力。我们通过荧光素酶和 Western blot 实验确定 SUFU 蛋白是 miR-224 的下游靶标。我们提出，miR-224 通过维持 Hedgehog 通路的活性促进 BC 细胞的生长和侵袭，而 SUFU 则负向调节该通路。综上所述，我们的研究表明 miR-224 可能在 BC 中作为一种致癌 miRNA 发挥作用，并提示 miR-224 可能是 BC 患者的一个潜在治疗靶点。
