Physikalisches Institut and CeNTech, Westfälische Wilhelms-Universität Münster, Heisenbergstraße 11, D-48149 Münster, Germany.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:125-130. doi: 10.1016/j.colsurfb.2017.07.062. Epub 2017 Jul 29.
A janus enantiomorphous nanomaterial assembly on substrate surfaces is prepared by depositing two different enantiomorphous nanomaterials (NMs) onto two different halves of the same glass substrate. This advanced construct allows us to simultaneously study and compare how the opposite biopolymer enantiomers functionalized onto the NM surfaces affect cell adhesion under exactly the same reaction conditions and at the same time, using a single biomaterial. Cell experiments show that cell adhesion to the Janus enantiomorphous NM assembly is chirality dependent when opposite enantiomers are examined on a single biomaterial. These results are more pronounced in serum-containing media, indicating the positive effect of protein adsorption on cell-chiral biomolecule functionalized NM surface interactions.
在基底表面制备手性二嵌段纳米材料组装体，是通过将两种不同的手性纳米材料（NMs）沉积到同一块玻璃基底的两个不同半块上实现的。这种先进的构建体使我们能够同时研究和比较在相同的反应条件下，功能化在 NM 表面上的相反生物聚合物对映异构体如何影响细胞黏附，同时使用单一生物材料。细胞实验表明，在手性二嵌段纳米材料组装体上检测到相反对映异构体时，细胞黏附与纳米材料的手性有关。在含有血清的培养基中，这些结果更为明显，表明蛋白质吸附对细胞-手性生物分子功能化 NM 表面相互作用的积极影响。
