Hanein D, Geiger B, Addadi L
Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
Science. 1994 Mar 11;263(5152):1413-6. doi: 10.1126/science.8128221.
Interactions during cell adhesion to external surfaces may reach the level of discrimination of molecular chirality. Cultured epithelial cells interact differently with the [011] faces of the (R,R) and (S,S) calcium tartrate tetrahydrate crystals. In a modified version of the classical Pasteur experiment, the enantiomorphous crystals were sorted out from a 1:1 mixture by the selective adhesion of cells to the (R,R) crystals. This stereospecificity results from molecular recognition between chiral components on the cell surface and the structured crystal surface. Crystals may allow experimental differentiation between distinct stages in cell substrate contacts, providing mechanistic information not readily attainable on conventional heterogeneous surfaces.
细胞与外部表面粘附过程中的相互作用可能达到分子手性识别的水平。培养的上皮细胞与（R，R）和（S，S）酒石酸钙四水合物晶体的[011]面相互作用方式不同。在经典巴斯德实验的一个改进版本中，通过细胞对（R，R）晶体的选择性粘附，从1:1混合物中筛选出对映形态晶体。这种立体特异性源于细胞表面手性成分与结构化晶体表面之间的分子识别。晶体可以使实验区分细胞与底物接触的不同阶段，提供在传统异质表面上不易获得的机制信息。
