Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Department of Fiber Science and Apparel Design, and Biomedical Engineering Field, Cornell University, Ithaca, NY, 14853-4401, USA.
Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:78-88. doi: 10.1016/j.colsurfb.2017.07.031. Epub 2017 Jul 29.
Selected from the family of self-designed biodegradable amino acid-based poly (ester urea urethane) (AA-PEUU) pseudo-protein biomaterials, arginine-leucine based poly (ester urea urethane)s (Arg-Leu-PEUUs) were used as protective and bio-functional coatings for bio-absorbable magnesium alloy MgZnYNd in cardiovascular stent applications. Comparing with poly (glycolide-co-lactide) (PLGA) coating, the Arg-Leu-PEUU coating had stronger bonding strength with the substrate; in vitro electrochemical and long-term immersion results verified a significantly better corrosion resistance. Acute blood contact tests proved a better hemocompatibility of Arg-Leu-PEUU coating. The immunofluorescent staining and cell proliferation test indicated that Arg-Leu-PEUU coating had a far better cytocompatibility. The Arg-Leu-PEUU coating stimulated human umbilical vein endothelial cells (HUVEC) to release reasonably increased amount of nitric oxide (NO), suggesting its potential in retarding thrombosis and restenosis. The superior corrosion resistance and biocompatibility as well as the indigenous NO production bio-functionality of the Arg-Leu-PEUU copolymer family indicate their capability to offer a far better protection of the magnesium-based implantable cardiovascular stent and bring their application closer to clinical reality.
从自主设计的可生物降解氨基酸基聚（酯脲）（AA-PEUU）伪蛋白生物材料家族中选择，精氨酸-亮氨酸基聚（酯脲）（Arg-Leu-PEUUs）被用作心血管支架应用中生物可吸收镁合金 MgZnYNd 的保护和生物功能涂层。与聚（乙交酯-co-丙交酯）（PLGA）涂层相比，Arg-Leu-PEUU 涂层与基体具有更强的结合强度；体外电化学和长期浸泡结果验证了其具有更好的耐腐蚀性。急性血液接触试验证明 Arg-Leu-PEUU 涂层具有更好的血液相容性。免疫荧光染色和细胞增殖试验表明 Arg-Leu-PEUU 涂层具有更好的细胞相容性。Arg-Leu-PEUU 涂层刺激人脐静脉内皮细胞（HUVEC）释放相当量增加的一氧化氮（NO），表明其在抑制血栓形成和再狭窄方面具有潜力。Arg-Leu-PEUU 共聚物家族的卓越耐腐蚀性和生物相容性以及其固有 NO 产生的生物功能表明，它们能够为基于镁的植入式心血管支架提供更好的保护，并使其应用更接近临床现实。
