College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong Province, 266003, PR China.
College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong Province, 266003, PR China.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:89-96. doi: 10.1016/j.colsurfb.2017.07.061. Epub 2017 Jul 26.
Nile tilapia skin collagen sponges were fabricated by freeze-drying technology and modified with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide in the presence of N-hydroxysuccinimide (EDC/NHS), genipin+PBS, genipin+ethanol, tea polyphenol (TP), nordihydroguaiaretic acid (NDGA) and diphenyl phosphoryl azide (DPPA). Physicochemical and biological properties, micromorphology and compatibility before and after modification were investigated to evaluate collagen sponge as a hemostatic biomedical material. The mechanical property of collagen sponges strengthened after cross-linking. The elongation at break of cross-linked collagen sponges decreased except for EDC/NHS, which was close to that of non-crosslinked. The collagen sponge cross-linked with EDC/NHS exhibited the highest hygroscopicity in comparison with other cross-linkers. The resistance to collagenase biodegradation of collagen sponges after cross-linking strengthened significantly except for NDGA. Collagen sponges cross-linked with EDC/NHS, TP and NDGA maintained high porosity (97-98%), similar to non-crosslinked (98.42%). Collagen sponges could shorten the blood coagulation time. From the variations of the FTIR spectrum pattern and SEM, DPPA could change the secondary structure of collagen and destroy the spongy structure of collagen sponge, which was not suitable for the cross-linking of collagen sponge. Whereas, EDC/NHS was recognized as a perfect cross-linker owing to its excellent properties and porous microstructure. All fabricated collagen sponges were recognized to be biocompatible by the hemolysis assay in vitro. Therefore, collagen sponge modified with EDC/NHS could be used as a perfect biomedical hemostatic material.
尼罗罗非鱼皮胶原海绵通过冷冻干燥技术制备，并在 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺存在下用 N-羟基琥珀酰亚胺（EDC/NHS）、京尼平+PBS、京尼平+乙醇、茶多酚（TP）、去甲二氢愈创木酸（NDGA）和二苯基磷酰叠氮（DPPA）进行修饰。研究了修饰前后的物理化学和生物学性质、微观形态和相容性，以评估胶原海绵作为止血生物医学材料的性能。交联后胶原海绵的力学性能增强。除 EDC/NHS 外，交联胶原海绵的断裂伸长率降低，接近未交联的。与其他交联剂相比，EDC/NHS 交联的胶原海绵吸湿性最高。除 NDGA 外，交联后胶原海绵对胶原酶生物降解的抵抗力显著增强。EDC/NHS、TP 和 NDGA 交联的胶原海绵保持了高孔隙率（97-98%），与未交联的相似（98.42%）。胶原海绵可以缩短凝血时间。从 FTIR 光谱模式和 SEM 的变化可以看出，DPPA 可以改变胶原的二级结构并破坏胶原海绵的海绵状结构，因此不适合胶原海绵的交联。而 EDC/NHS 被认为是一种理想的交联剂，因为它具有优良的性能和多孔的微观结构。所有制备的胶原海绵在体外溶血试验中均被认为具有良好的生物相容性。因此，用 EDC/NHS 修饰的胶原海绵可用作理想的生物医学止血材料。
