College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong 266003, PR China.
College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao, Shandong 266003, PR China.
J Mech Behav Biomed Mater. 2018 Apr;80:51-58. doi: 10.1016/j.jmbbm.2018.01.006. Epub 2018 Jan 10.
The objective of this study was to explore the effects of dehydrothermal treatment (DHT) and glutaraldehyde (GTA) cross-linking on mechanical, biological properties and biodegradation behavior of Nile tilapia skin collagen sponge fabricated by freeze-drying technology. It was found that the GTA cross-linked collagen sponge exhibited a higher degree of cross-linking in comparison with DHT. The extent of increased tensile strength as well as hygroscopicity indicated that GTA cross-linking was superior to DHT in mechanical properties and liquid absorption, which was attributed to different cross-linking mechanisms. Hygroscopicity assay indicated that cross-linking could improve stability of collagen in solutions. No obvious changes in porosity and blood coagulation time were observed whether cross-linking or not. Results from collagenase biodegradation assay in vitro illustrated that GTA-treated collagen sponge was more resistant to collagenase biodegradation, while DHT exhibited negligible resistance. In addition, photochemical stability of collagen sponge was studied by Fourier transforms infrared spectroscopy (FTIR), which indicated that both cross-linking treatments could not change the backbone structure of collagen. Furthermore, the microstructure of collagen sponge was stable after cross-linking. The highly porous and interconnected structure of collagen sponge was helpful to the absorption of wound exudates, supplement of oxygen and cell proliferation, accompanied with good blood compatibility, which indicated that our fabricated collagen sponge could be applied in biomedical materials field as wound dressings.
本研究旨在探讨去水热处理（DHT）和戊二醛（GTA）交联对通过冷冻干燥技术制备的尼罗罗非鱼皮胶原蛋白海绵的机械性能、生物学性能和生物降解行为的影响。结果发现，与 DHT 相比，GTA 交联的胶原蛋白海绵具有更高的交联度。拉伸强度和吸湿性的增加程度表明，GTA 交联在机械性能和液体吸收方面优于 DHT，这归因于不同的交联机制。吸湿性试验表明，交联可以提高胶原蛋白在溶液中的稳定性。无论交联与否，孔隙率和凝血时间均无明显变化。体外胶原酶降解试验结果表明，经 GTA 处理的胶原蛋白海绵对胶原酶的降解更具抵抗力，而 DHT 则几乎没有抵抗力。此外，通过傅里叶变换红外光谱（FTIR）研究了胶原海绵的光化学稳定性，结果表明两种交联处理均不能改变胶原的骨架结构。此外，交联后胶原海绵的微观结构稳定。胶原海绵具有高度多孔和相互连接的结构，有助于吸收伤口渗出物、补充氧气和促进细胞增殖，同时具有良好的血液相容性，这表明我们制备的胶原海绵可以作为伤口敷料应用于生物医学材料领域。
