Uhlig Silvio, Stanic Ana, Hussain Fozia, Miles Christopher O
Norwegian Veterinary Institute, P.O. Box 750 Sentrum, 0106, Oslo, Norway.
Norwegian Veterinary Institute, P.O. Box 750 Sentrum, 0106, Oslo, Norway.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:322-326. doi: 10.1016/j.jchromb.2017.07.017. Epub 2017 Jul 11.
Commercial immunoaffinity columns (IACs) are today available for all major mycotoxins. However, manufacturers give usually no or very limited information on the epitope, i.e. the specific part of the toxin molecule that binds to the antibody. 4-Deoxynivalenol (DON) is a trichothecene mycotoxin that is produced by plant pathogenic field fungi and is regulated in many countries worldwide. DON was shown to be biotransformed via different metabolic pathways, and thus many different biotransformation products may be found in different products or organisms. In addition, several structurally similar mycotoxins may co-occur with DON. We compared five commercial IACs for their retention of a range of DON derivatives modified in the C-3, C-8, C-10, C-13 or C-15 positions, as well as nivalenol (NIV) and T-2 tetraol. The DON-derivatives were deepoxy-DON, DON 3-, 8- and 15-O-β-d-glucuronides, 3- and 15-O-acetyl-DON, DON-3-O-β-d-glucoside, 10- and 13-cysteinyl-adducts of DON, and the 13-mercaptoethanol and 10,13-dimercaptoethanol adducts of DON. The C-3 derivatives and deepoxy-DON were retained by most of the columns. Only one of the five IACs retained C-15 and C-8 derivatives, but it did not retain C-3 derivatives or deepoxy-DON. The antibodies in two of the IACs bound C-10 conjugates, but C-13 derivatives were not retained by any of the columns. This study shows that all of the antibodies in commercial IACs bind a range of DON derivatives, especially those that are modified at C-3. NIV was retained by three of the columns, and T-2 tetraol was partially retained by one IAC.
如今，市面上有针对所有主要霉菌毒素的商用免疫亲和柱（IAC）。然而，制造商通常提供的关于表位的信息很少或非常有限，表位即毒素分子中与抗体结合的特定部分。4-脱氧雪腐镰刀菌烯醇（DON）是一种单端孢霉烯族霉菌毒素，由植物病原性田间真菌产生，在世界许多国家都受到监管。DON已被证明可通过不同的代谢途径进行生物转化，因此在不同的产品或生物体中可能会发现许多不同的生物转化产物。此外，几种结构相似的霉菌毒素可能与DON同时出现。我们比较了五种商用IAC对一系列在C-3、C-8、C-10、C-13或C-15位置修饰的DON衍生物以及雪腐镰刀菌烯醇（NIV）和T-2四醇的保留情况。DON衍生物包括去环氧-DON、DON 3-、8-和15-O-β-D-葡萄糖醛酸苷、3-和15-O-乙酰基-DON、DON-3-O-β-D-葡萄糖苷、DON的10-和13-半胱氨酸加合物以及DON的13-巯基乙醇和10,13-二巯基乙醇加合物。大多数柱子都能保留C-3衍生物和去环氧-DON。五个IAC中只有一个保留了C-15和C-8衍生物，但它不保留C-3衍生物或去环氧-DON。两个IAC中的抗体能结合C-10缀合物，但所有柱子都不保留C-13衍生物。这项研究表明，商用IAC中的所有抗体都能结合一系列DON衍生物，尤其是那些在C-3位置修饰的衍生物。三种柱子能保留NIV，一种IAC能部分保留T-2四醇。
