Department of Food Science and Technology, HungKuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung City, 43302, Taiwan, Republic of China.
Department of Food and Nutrition, Providence University, Taichung City, 43301, Taiwan, Republic of China.
Probiotics Antimicrob Proteins. 2018 Jun;10(2):210-217. doi: 10.1007/s12602-017-9302-x.
Urinary tract infections (UTIs) are the most common infectious diseases in infants and the elderly; they are also the most common among nosocomial infections. The treatment of UTIs usually involves a short-term course of antibiotics. The purpose of this study was to identify the strains of lactic acid bacteria (LAB) that can inhibit the urinary tract pathogen Staphylococcus saprophyticus, as alternatives to antibiotics. In this study, we collected 370 LAB strains from fermented plant products and reference strains from the Bioresources Collection and Research Center (BCRC). Using spent culture supernatants (SCS), we then screened these LAB strains with for antimicrobial effects on urinary tract pathogens by the well-diffusion assay. Seven LAB strains-PM2, PM68, PM78, PM201, PM206, PM229, and RY2-exhibited inhibitory activity and were evaluated for anti-growth activity against urinary tract pathogens by the co-culture inhibition assay. Anti-adhesion and anti-invasion activities against urinary tract pathogens were evaluated using the SV-HUC-1 urothelial cell cultures. The results revealed that the survival rate of S. saprophyticus ranged from 0.9-2.96%, with the pH continuously decreasing after co-culture with LAB strains for 4 h. In the competitive adhesion assay, the exclusion and competition groups performed better than the displacement group. In the SV-HUC-1 cell invasion assay, PM201, PM206, PM229, and RY2 were found to inhibit the invasion of SV-HUC-1 cells by S. saprophyticus BCRC 10786. To conclude, RY2, PM229, and PM68 strains exhibited inhibitory activity against the urinary tract pathogen S. saprophyticus.
尿路感染（UTI）是婴儿和老年人中最常见的传染病；它们也是医院获得性感染中最常见的传染病。UTI 的治疗通常涉及短期使用抗生素。本研究旨在鉴定出可以抑制尿路病原体腐生葡萄球菌的乳酸菌（LAB）菌株，作为抗生素的替代品。在本研究中，我们从发酵植物产品和生物资源收集与研究中心（BCRC）的参考菌株中收集了 370 株 LAB 菌株。使用废弃培养上清液（SCS），我们通过平板扩散法筛选这些 LAB 菌株对尿路病原体的抗菌作用。有 7 株 LAB 菌株-PM2、PM68、PM78、PM201、PM206、PM229 和 RY2-表现出抑制活性，并通过共培养抑制试验评估了对尿路病原体的生长抑制活性。通过 SV-HUC-1 尿路上皮细胞培养物评估对尿路病原体的抗粘附和抗侵袭活性。结果表明，腐生葡萄球菌的存活率为 0.9-2.96%，与 LAB 菌株共培养 4 小时后 pH 值持续下降。在竞争粘附试验中，排除和竞争组的表现优于置换组。在 SV-HUC-1 细胞侵袭试验中，发现 PM201、PM206、PM229 和 RY2 抑制了腐生葡萄球菌 BCRC 10786 对 SV-HUC-1 细胞的侵袭。总之，RY2、PM229 和 PM68 菌株对尿路病原体腐生葡萄球菌具有抑制活性。
