天然细菌成因氧化铁中亚微米尺度下微生物周围铁物种的空间分辨分布
Spatially Resolved Distribution of Fe Species around Microbes at the Submicron Scale in Natural Bacteriogenic Iron Oxides.
作者信息
Suga Hiroki, Kikuchi Sakiko, Takeichi Yasuo, Miyamoto Chihiro, Miyahara Masaaki, Mitsunobu Satoshi, Ohigashi Takuji, Mase Kazuhiko, Ono Kanta, Takahashi Yoshio
机构信息
Department of Earth and Planetary Systems Science, Graduate School of Science (DEPSS), Hiroshima University.
Project Team for Development of New-Generation Research Protocol for Submarine Resources, Japan Agency for Marine-Earth Science and Technology (JAMSTEC).
出版信息
Microbes Environ. 2017 Sep 27;32(3):283-287. doi: 10.1264/jsme2.ME17009. Epub 2017 Aug 5.
Natural bacteriogenic iron oxides (BIOS) were investigated using local-analyzable synchrotron-based scanning transmission X-ray microscopy (STXM) with a submicron-scale resolution. Cell, cell sheath interface (EPS), and sheath in the BIOS were clearly depicted using C-, N-, and O- near edge X-ray absorption fine structure (NEXAFS) obtained through STXM measurements. Fe-NEXAFS obtained from different regions of BIOS indicated that the most dominant iron mineral species was ferrihydrite. Fe(II)- and/or Fe(III)-acidic polysaccharides accompanied ferrihydrite near the cell and EPS regions. Our STXM/NEXAFS analysis showed that Fe species change continuously between the cell, EPS, and sheath under several 10-nm scales.
利用具有亚微米级分辨率的基于同步加速器的局部可分析扫描透射X射线显微镜（STXM）对天然细菌生成的铁氧化物（BIOS）进行了研究。通过STXM测量获得的C、N和O近边X射线吸收精细结构（NEXAFS）清晰地描绘了BIOS中的细胞、细胞鞘界面（EPS）和鞘。从BIOS不同区域获得的Fe-NEXAFS表明，最主要的铁矿物种类是水铁矿。在细胞和EPS区域附近，Fe（II）和/或Fe（III）酸性多糖与水铁矿相伴。我们的STXM/NEXAFS分析表明，在几十纳米的尺度下，细胞、EPS和鞘之间的Fe物种会持续变化。
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