Sun Lindan, Tu Jiagang, Yi Lizhu, Chen Wenjie, Zhao Lijuan, Huang Yunmao, Liang Rishen, Li Jun, Zhou Meng, Lin Li
Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Microb Pathog. 2017 Sep;110:578-585. doi: 10.1016/j.micpath.2017.07.042. Epub 2017 Aug 3.
Snakehead vesiculovirus (SHVV) has caused mass mortality to cultured snakehead fish in China, resulting in enormous economic losses in snakehead fish culture. In this report, the whole genome of SHVV was sequenced. Interestingly, it shared more than 94% nucleotide sequence identity with Monopterus albus rhabdovirus (MoARV), which has caused great economic loss to cultured rice field eel (Monopterus albus). Therefore, the concern of cross-species infection of these viruses prompted us to investigate the susceptibility of rice field eel to SHVV infection. The results showed that rice field eel was susceptible to SHVV in both intracoelomical injection and immersion routes. Severe hemorrhage was observed on the skin and visceral organs of SHVV-infected rice field eels. Histopathological examination showed vacuoles in the tissues of infected liver, kidney and heart. Viral RNA or protein was detected in the tissues of infected fish by reverse transcription polymerization chain reaction (RT-PCR), in situ hybridization (ISH), or immunohistochemistry assay (IHC). Investigation of the epidemic of vesiculovirus in rice field eel as well as other co-cultured fish is invaluable for the prevention of vesiculovirus infection.
乌鳢水泡病毒（SHVV）已在中国导致养殖乌鳢大量死亡，给乌鳢养殖造成巨大经济损失。在本报告中，对SHVV的全基因组进行了测序。有趣的是，它与黄鳝弹状病毒（MoARV）的核苷酸序列同一性超过94%，MoARV已给养殖黄鳝（Monopterus albus）造成巨大经济损失。因此，对这些病毒跨物种感染的担忧促使我们研究黄鳝对SHVV感染的易感性。结果表明，黄鳝通过体腔注射和浸泡途径均对SHVV易感。在感染SHVV的黄鳝的皮肤和内脏器官上观察到严重出血。组织病理学检查显示，感染的肝脏、肾脏和心脏组织中出现空泡。通过逆转录聚合酶链反应（RT-PCR）、原位杂交（ISH）或免疫组织化学分析（IHC）在感染鱼的组织中检测到病毒RNA或蛋白质。调查黄鳝以及其他混养鱼类中水泡病毒的流行情况对于预防水泡病毒感染具有重要价值。
