Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding; Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China.
Virology. 2019 Jan 2;526:32-37. doi: 10.1016/j.virol.2018.10.002. Epub 2018 Oct 15.
Snakehead vesiculovirus (SHVV) is a new rhabdovirus isolated from diseased hybrid snakehead fish (Channa maculate ♀ x Channa argus ♂) and has caused serious economic losses in snakehead fish culture in China. To better understand the pathogenicity of SHVV, we developed a reverse genetics system for SHVV by using human and fish cells. In detail, human 293T cells were co-transfected with four plasmids encoding the full-length SHVV antigenomic RNA or the supporting proteins including nucleoprotein (N), phosphoprotein (P), and large polymerase (L), followed by the cultivation in Channel catfish ovary (CCO) cells. We also rescued a recombinant SHVV expressing enhanced green fluorescent protein (EGFP), which was inserted into the 3' non-coding region (NCR) of the glycoprotein (G) gene of SHVV. Our study provides a potential tool for unveiling the pathogenicity of SHVV and a template for the rescue of other fish viruses by using both human 293T and fish cells.
蛇头鱼虹彩病毒（SHVV）是一种从患病杂交蛇头鱼（♀斑鳢×♂乌鳢）中分离出来的新型弹状病毒，在中国的蛇头鱼养殖中造成了严重的经济损失。为了更好地了解 SHVV 的致病性，我们使用人和鱼细胞开发了 SHVV 的反向遗传学系统。具体来说，用人 293T 细胞共转染了四个质粒，分别编码全长 SHVV 抗原基因组 RNA 或支持蛋白，包括核蛋白（N）、磷蛋白（P）和大聚合酶（L），然后在斑点叉尾鮰卵巢（CCO）细胞中培养。我们还拯救了一株表达增强型绿色荧光蛋白（EGFP）的重组 SHVV，该蛋白插入 SHVV 的糖蛋白（G）基因的 3'非编码区（NCR）。我们的研究为揭示 SHVV 的致病性提供了一个潜在的工具，并为使用人和鱼细胞拯救其他鱼类病毒提供了一个模板。
