Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha, China.
Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha, China.
Biomed Pharmacother. 2017 Oct;94:612-618. doi: 10.1016/j.biopha.2017.07.111. Epub 2017 Aug 4.
Long non-coding RNA nuclear-enriched abundant transcript 1 (NEAT1) was found to be participated in tumorigenesis in various cancers including hepatocellular carcinoma (HCC). However, the clinical implication and underling function of NEAT1 in HCC have not been fully known.
The relative NEAT1 expression was examined by qRT-PCR in HCC tissues and cells, compared with adjacent normal tissues and normal human hepatocyte (LO2) cells, respectively. Cell proliferation and invasion were examined by MTT, cell colony formation and transwell assays. Luciferase reporter assay was performed to verify miR-613 was a direct target of NEAT1. Western blot analysis was also performed.
NEAT1 was notably upregulated in HCC tissues and cells. Higher NEAT1 expression associated with larger tumor size and vascular invasion of HCC patients. Knockdown of NEAT1 significantly suppressed HCC cell proliferation, colony formation and cell invasion. Interestingly, lower miR-613 expression negatively associated with the NEAT1 expression in HCC tissues and was regulated by NEAT1. Additionally, we demonstrated that NEAT1 promoted HCC cell proliferation and invasion by regulating miR-613 expression.
These results implied that inhibition of NEAT1 may be a potential therapeutic strategy for HCC patients.
长链非编码 RNA 核丰富丰富转录本 1（NEAT1）被发现参与多种癌症的肿瘤发生，包括肝细胞癌（HCC）。然而，NEAT1 在 HCC 中的临床意义和潜在功能尚未完全了解。
通过 qRT-PCR 分别在 HCC 组织和细胞中检测相对 NEAT1 表达，与相邻正常组织和正常人肝细胞（LO2）细胞进行比较。通过 MTT、细胞集落形成和 Transwell 测定分别检测细胞增殖和侵袭。通过荧光素酶报告基因测定验证 miR-613 是 NEAT1 的直接靶标。还进行了 Western blot 分析。
NEAT1 在 HCC 组织和细胞中明显上调。较高的 NEAT1 表达与 HCC 患者的肿瘤大小较大和血管侵袭相关。敲低 NEAT1 可显著抑制 HCC 细胞的增殖、集落形成和细胞侵袭。有趣的是，较低的 miR-613 表达与 HCC 组织中的 NEAT1 表达呈负相关，并且受到 NEAT1 的调节。此外，我们证明 NEAT1 通过调节 miR-613 表达促进 HCC 细胞的增殖和侵袭。
这些结果表明抑制 NEAT1 可能是 HCC 患者的一种潜在治疗策略。
