Smirnova N S, Popov V L, Kokorin I N
Acta Virol. 1986 Sep;30(5):436-9.
Ultrastructure of Rickettsia prowazekii has been followed in L-929 cells 4 days post-infection (p.i.) by cryoultramicrotomy. Groups of rickettsiae were present in the cytoplasm outside of vacuoles forming microcolonies. The size of rickettsiae amounted to 400 X 700 nm, the average thickness of the cell wall was 5 nm, that of periplasmic space and cytoplasmic membrane 14 and 6 nm, respectively. Within intracytoplasmic colonies the rickettsiae were tightly packed and their cell walls were closely adjacent to each other. No halo or capsule-like coating around them was detected. No ultrastructural details were observed in the light translucent spaces between cells. Marginal rickettsiae of the microcolonies were often in close contact with the host cell mitochondria.
通过冷冻超薄切片术，在感染普氏立克次体4天后的L-929细胞中观察其超微结构。空泡外的细胞质中存在立克次体群，形成微菌落。立克次体大小为400×700纳米，细胞壁平均厚度为5纳米，周质空间和细胞质膜的平均厚度分别为14纳米和6纳米。在胞内菌落中，立克次体紧密堆积，它们的细胞壁彼此紧密相邻。未检测到它们周围有晕圈或胶囊样包膜。在细胞间的透光空间中未观察到超微结构细节。微菌落边缘的立克次体常与宿主细胞线粒体紧密接触。
