Popov V L, Dyuisalieva R G, Smirnova N S, Tarasevich I V, Rybkin N N
Acta Virol. 1986 Nov;30(6):494-8.
Rickettsia sibirica (strain Netsvetaev) was found within large translucent spaces in the cytoplasm of L-929 cell monolayer cultures on day 6 postinfection (p.i.). These rickettsiae were rod shaped 0.3 X 10 microns in size encircled by a halo of up to 200 nm wide corresponding to a capsule-like coat. Directly on the cell wall was a 12 nm thick microcapsule in which subunit structures with 12 nm thick spacing could be recognized. The cell wall membrane was 14 nm thick with a wider internal layer occasionally in a section found split into two electron-dense lamels; the internal layer corresponded to peptidoglycan. The periplasmic space with an average thickness of 5 nm separated the cell wall from a 7 nm thick cytoplasmic membrane. The ultrastructure of R. sibirica was similar to that of other rickettsiae, although the capsule-like coat was thicker than in spotted fever (SF) group rickettsiae.
感染后第6天，在L-929细胞单层培养物的细胞质中，于大的半透明区域内发现了西伯利亚立克次体（涅斯韦塔耶夫菌株）。这些立克次体呈杆状，大小为0.3×10微米，周围有一圈宽达200纳米的光晕，对应于类似荚膜的包膜。紧贴细胞壁的是一层12纳米厚的微荚膜，其中可识别出间距为12纳米的亚基结构。细胞壁膜厚14纳米，其较宽的内层在切片中偶尔可见分裂成两个电子致密层；内层对应于肽聚糖。平均厚度为5纳米的周质空间将细胞壁与7纳米厚的细胞质膜分隔开。西伯利亚立克次体的超微结构与其他立克次体相似，尽管其类似荚膜的包膜比斑点热（SF）群立克次体的更厚。
