Popov V L, Prozorovsky S V, Vovk O A, Kekcheeva N K, Smirnova N S, Barkhatova O I
Acta Virol. 1987 Jan;31(1):53-8.
Monolayer cultures of peritoneal macrophages of intact guinea pigs were infected with Rickettsia prowazekii (strain Breinl) and examined by electron microscopy after 30 min, 4 and 24 hr post-infection (p.i.). Three parallel processes developed in infected macrophages: reproduction of rickettsiae in macrophage cytoplasm, destruction in phagolysosomes and production of spheroplast-like forms. Reproduction of rickettsiae yielded 2 cell types: those with dense and with light cytoplasm; they were located side by side in the microcolony and seemed to have a common capsule-like coat. Relatively small spheroplast-like forms of about 1 micron in size were regularly detected. Addition of immune serum to macrophages increased the number of rickettsiae, both of rod-shaped as well as of spheroplast-like ones located within phagosomes, but elicited no increase in the number of digested pathogen cells.
用普氏立克次体（布氏菌株）感染健康豚鼠的腹膜巨噬细胞单层培养物，并在感染后30分钟、4小时和24小时通过电子显微镜检查。受感染的巨噬细胞出现了三个平行过程：立克次体在巨噬细胞胞质内繁殖、在吞噬溶酶体中被破坏以及产生球形体样形态。立克次体繁殖产生了两种细胞类型：胞质致密的和胞质浅色的；它们在微菌落中并排排列，似乎有一层共同的胶囊样包膜。经常检测到相对较小的约1微米大小的球形体样形态。向巨噬细胞中添加免疫血清增加了吞噬体内杆状和球形体样立克次体的数量，但未引起被消化病原体细胞数量的增加。
