Kassem Hassan E, Talaat Iman M, El-Sawa Afaf, Ismail Hanan, Zaher Abbas
Department of Orthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Eur J Oral Sci. 2017 Oct;125(5):361-370. doi: 10.1111/eos.12366. Epub 2017 Aug 8.
We investigated the effect of different force magnitudes on osteocyte apoptosis in a model of orthodontic tooth movement. Forty-nine male Sprague Dawley rats (7-9 wk of age) were divided into light- and heavy-force groups (n = 21 each group) and a control group (n = 7). A coil spring delivered pressure (either 10-15 g or 20-25 g) to the left maxillary first molar. The rats were sacrificed 1, 3, or 5 d after placement of the appliance. Sections of the maxillary first molars were immunostained for caspase-3. Upon force application, the number of apoptotic osteocytes significantly increased in the pressure side at 1 d and remained the same at 3 d and 5 d. However, there was no significant difference in the number of apoptotic osteocytes between the two force groups. We conclude that osteocyte apoptosis appears to increase under orthodontic loading, reaching a plateau after 1 d. However, osteocyte apoptosis seems to be independent of the magnitude of orthodontic forces tested.
我们在正畸牙齿移动模型中研究了不同力值对骨细胞凋亡的影响。将49只雄性Sprague Dawley大鼠（7 - 9周龄）分为轻力组和重力组（每组n = 21）以及对照组（n = 7）。用螺旋弹簧向左侧上颌第一磨牙施加压力（10 - 15克或20 - 25克）。在放置矫治器后1、3或5天处死大鼠。对上颌第一磨牙切片进行半胱天冬酶-3免疫染色。施加力后，压力侧凋亡骨细胞数量在1天时显著增加，在3天和5天时保持不变。然而，两个力组之间凋亡骨细胞数量没有显著差异。我们得出结论，在正畸加载下骨细胞凋亡似乎会增加，1天后达到平台期。然而，骨细胞凋亡似乎与所测试的正畸力大小无关。
