Li Mengshuang, Lu Jing, Tao Mingmin, Li Mengru, Yang Hua, Xia En-Hua, Chen Qi, Wan Xiaochun
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China.
College of Science, Anhui Agricultural University, Hefei, China.
Front Plant Sci. 2020 Sep 4;11:544933. doi: 10.3389/fpls.2020.544933. eCollection 2020.
Polyamines (PAs) in plant play a critical role in growth and development and in response to environmental stress. Polyamine oxidase (PAO) is a flavin adenine dinucleotide dependent enzyme that plays a major role in PA catabolism. For the first time, genes in tea plant were screened for the whole genome-wide and seven genes were identified, which were named . Phylogenetic tree analysis revealed seven CsPAO protein sequences classed into three groups, including clade I, III, and IV. Compared with other plants, the tea plant lacked clade II members. Genetic structure and tissue specific expression analysis showed that there were significant differences among members of the gene family. Among members of the family, and contain more introns and are highly expressed in various organizations. , , and genes were cloned and expressed heterologously to verify theirs function. Heat map showed high response of to drought stress, while 1 and 2 were sensitive to changes in nitrogen nutrition. Furthermore, exogenous abscisic acid (ABA) treatment indicated that the expression of most genes in roots and leaves was significantly induced. In the root, Spm content increased significantly, while Put and Spd content decreased, suggesting that ABA has great influence on the biosynthesis of PAs. Anaerobic treatment of picked tea leaves showed that the decomposition of PAs was promoted to a certain extent. The above data help to clarify the role of in response abiotic and nitrogen nutritional stresses in tea plants, and provide a reference perspective for the potential influence of PAs on the tea processing quality.
植物中的多胺（PAs）在生长发育以及对环境胁迫的响应中起着关键作用。多胺氧化酶（PAO）是一种黄素腺嘌呤二核苷酸依赖性酶，在多胺分解代谢中起主要作用。首次在茶树全基因组范围内筛选基因，鉴定出7个基因，并对其进行了命名。系统发育树分析表明，7个CsPAO蛋白序列分为三组，包括进化枝I、III和IV。与其他植物相比，茶树缺乏进化枝II成员。基因结构和组织特异性表达分析表明，该基因家族成员之间存在显著差异。在该家族成员中，[具体基因名称1]和[具体基因名称2]含有更多内含子，并且在各种组织中高表达。[具体基因名称3]、[具体基因名称4]和[具体基因名称5]基因被克隆并进行异源表达以验证其功能。热图显示[具体基因名称6]对干旱胁迫反应强烈，而[具体基因名称7]和[具体基因名称8]对氮营养变化敏感。此外，外源脱落酸（ABA）处理表明，根和叶中大多数[具体基因名称]基因的表达均被显著诱导。在根中，亚精胺（Spm）含量显著增加，而腐胺（Put）和精胺（Spd）含量降低，这表明ABA对多胺的生物合成有很大影响。对采摘的茶叶进行厌氧处理表明，多胺的分解在一定程度上得到了促进。上述数据有助于阐明[具体基因名称]在茶树对非生物和氮营养胁迫响应中的作用，并为多胺对茶叶加工品质的潜在影响提供参考视角。
