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大鼠腮腺中α-淀粉酶昼夜节律的个体发生

Ontogeny of alpha-amylase circadian rhythms in rat parotid gland.

作者信息

Sanz E G, Vermouth N T, Bellavia S L

出版信息

Comp Biochem Physiol A Comp Physiol. 1986;85(3):571-4. doi: 10.1016/0300-9629(86)90448-2.

DOI:10.1016/0300-9629(86)90448-2
PMID:2878787
Abstract

The content of alpha-amylase (alpha-1,4-glucan-4-glucanohydrolase, EC 3.2.1.1.) and total soluble proteins of parotid glands (from rats exposed to a photoperiod of 14 hr light: 10 hr dark), have been determined every 2 or 3 hr over 24 hr periods in 15, 25 and 90-day-old rats. In 35-, 45- and 72-day-old rats, determinations were performed only at 0100 and 1400 hr. The alpha-amylase and total soluble protein contents from 90-day-old rats show a circadian variation, with a maximum value at 2200 hr and a minimum at 1400 hr. Parotids from 15- and 25-day-old rats also show a circadian rhythm. The minimum value is recorded at 0100 hr and the maximum at 1400 hr. At day 35 and after, there is an inversion of the amylase rhythm. In immature rats, it appears that alpha-amylase and soluble protein are under the influence of another synchronizer, whose timing is independent of that imposed by mastication of solid food.

摘要

在15日龄、25日龄和90日龄大鼠中,每隔2或3小时测定一次(暴露于14小时光照:10小时黑暗光周期的大鼠)腮腺中α-淀粉酶(α-1,4-葡聚糖-4-葡聚糖水解酶,EC 3.2.1.1.)和总可溶性蛋白的含量,持续24小时。在35日龄、45日龄和72日龄大鼠中,仅在01:00和14:00进行测定。90日龄大鼠的α-淀粉酶和总可溶性蛋白含量呈现昼夜变化,在22:00达到最大值,在14:00达到最小值。15日龄和25日龄大鼠的腮腺也呈现昼夜节律。最小值出现在01:00,最大值出现在14:00。在35日龄及之后,淀粉酶节律出现反转。在未成熟大鼠中,α-淀粉酶和可溶性蛋白似乎受另一种同步器的影响,其时间安排独立于固体食物咀嚼所施加的时间。

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