Robinson Sara C, Hinsch Eric, Weber Genevieve, Leipus Kristina, Cerney Daniel
Department of Wood Science & Engineering, 119 Richardson Hall, Oregon State University, Corvallis, OR 97331, USA.
School of Forest Resources and Environmental Science, Michigan Technological University, 1400 Townsend Dr., Houghton, MI 49931, USA.
Materials (Basel). 2014 Jul 24;7(8):5427-5437. doi: 10.3390/ma7085427.
The extracellular colorants produced by , , and , three commonly utilized spalting fungi, were tested against a standard woodworker's aniline dye to determine if the fungal colorants could be utilized in an effort to find a naturally occurring replacement for the synthetic dye. Fungal colorants were delivered in two methods within a pressure treater-the first through solubilization of extracted colorants in dichloromethane, and the second via liquid culture consisting of water, malt, and the actively growing fungus. Visual external evaluation of the wood test blocks showed complete surface coloration of all wood species with all colorants, with the exception of the green colorant (xylindein) from in liquid culture, which did not produce a visible surface color change. The highest changes in external color came from noble fir, lodgepole pine, port orford cedar and sugar maple with aniline dye, cottonwood with the yellow colorant in liquid culture, lodgepole pine with the red colorant in liquid culture, red alder and Oregon maple with the green colorant in dichloromethane, and sugar maple and port orford cedar with the yellow colorant in dichloromethane. The aniline dye was superior to the fungal colorants in terms of internal coloration, although none of the tested compounds were able to completely visually color the inside of the test blocks.
对三种常用的变色真菌——[具体真菌名称1]、[具体真菌名称2]和[具体真菌名称3]产生的细胞外色素，与一种标准的木工苯胺染料进行了测试，以确定这些真菌色素是否可用于寻找合成染料的天然替代品。真菌色素通过两种方法在压力处理机中施加——第一种是将提取的色素溶解在二氯甲烷中，第二种是通过由水、麦芽和活跃生长的真菌组成的液体培养物。对木材测试块的外观视觉评估显示，除了液体培养物中[具体真菌名称3]产生的绿色色素（木素）外，所有木材种类用所有色素处理后表面均完全着色，该绿色色素未产生可见的表面颜色变化。外观颜色变化最大的是：用苯胺染料处理的高贵冷杉、黑松、俄勒冈白栎和糖枫；用液体培养物中的黄色色素处理的三角叶杨；用液体培养物中的红色色素处理的黑松；用二氯甲烷中的绿色色素处理的红桤木和俄勒冈枫香；以及用二氯甲烷中的黄色色素处理的糖枫和俄勒冈白栎。就内部着色而言，苯胺染料优于真菌色素，尽管所测试的化合物均无法使测试块内部完全在视觉上着色。
