Zschätzsch Marlen, Steudler Susanne, Reinhardt Olena, Bergmann Pia, Ersoy Franziska, Stange Stephanie, Wagenführ André, Walther Thomas, Berger Ralf Günter, Werner Anett
Institute of Natural Materials Technology Chair of Bioprocess Engineering Faculty of Mechanical Engineering Technical University of Dresden Dresden Germany.
Institute of Natural Materials Technology Chair of Wood Technology and Fibre Materials Technology Technical University of Dresden Dresden Germany.
Eng Life Sci. 2021 Jan 26;21(3-4):270-282. doi: 10.1002/elsc.202000079. eCollection 2021 Mar.
The replacement of potentially hazardous synthetic dyes with natural dyes and pigments are of great interest for a sustainable economy. In order to obtain cost-efficient, environmentally friendly and competitive products, improvements in the cultivation and extraction of pigment-producing organisms and in dyeing processes are necessary. In our study, we were able to scale up the production of xylindein by from 3 to 70 L bioreactor cultivations. We have identified important bioprocess parameters like low shear stress (150 rpm, tip speed <0.5 m/s) for optimal pigment yield (4.8 mg/L/d). Additionally, we have demonstrated the potential of laetiporic acid production by in various cultivation systems and media, achieving dried biomass concentrations of almost 10 g/L with a 7 L bioreactor cultivation after 17 days. Extractions performed at 70°C and 15 min incubation time showed optimal results. To the best of our knowledge, we have described for the first time the use of this pigment in silk dyeing, which results in a brilliant hue that cannot easily be produced by other natural pigments.
用天然染料和颜料替代潜在危险的合成染料对可持续经济具有重大意义。为了获得具有成本效益、环境友好且具有竞争力的产品，有必要改进色素生产生物的培养和提取以及染色工艺。在我们的研究中，我们能够通过3至70升生物反应器培养将木林菌素的产量扩大规模。我们已经确定了重要的生物工艺参数，如低剪切应力（150转/分钟，叶尖速度<0.5米/秒）以实现最佳色素产量（4.8毫克/升/天）。此外，我们已经证明了在各种培养系统和培养基中生产乳菇酸的潜力，在17天后通过7升生物反应器培养实现了近10克/升的干生物量浓度。在70°C和15分钟孵育时间下进行的提取显示出最佳结果。据我们所知，我们首次描述了这种色素在丝绸染色中的应用，其产生的鲜艳色调不易由其他天然色素产生。
