Nakamura H, Tanaka Y, Komuro-Tsujimoto A, Ishikawa K, Takadaya K, Tozawa H, Tsujimoto H, Honjo S, Hayami M
Int J Cancer. 1986 Dec 15;38(6):867-75. doi: 10.1002/ijc.2910380614.
Cynomolgus monkeys and squirrel monkeys were inoculated with autologous lymphoid cell lines immortalized by and producing human T-cell leukemia virus type-I (HTLV-I) in order to serve as an animal model of adult T-cell leukemia (ATL). The autologous cell lines were established from peripheral blood mononuclear cells (PBMC) from each monkey by co-cultivation with lethally irradiated MT-2 cells producing HTLV-I. All of these cell lines, which had monkey karyotypes, grew continuously without addition of interleukin-2 (IL-2) and expressed virus-specific proteins of HTLV-I and IL-2 receptor. After inoculation with the autologous cell lines, specific antibodies against HTLV-I proteins could be detected in their plasma, and transformed HTLV-I-infected cells could be recovered from their peripheral blood for at least 6 months. However, no signs of ATL have been observed to data, i.e. 2 years after inoculation.
将食蟹猴和松鼠猴接种由人类I型T细胞白血病病毒（HTLV-I）永生化并产生该病毒的自体淋巴样细胞系，以作为成人T细胞白血病（ATL）的动物模型。通过与经致死剂量照射的产生HTLV-I的MT-2细胞共培养，从每只猴子的外周血单个核细胞（PBMC）建立自体细胞系。所有这些具有猴子核型的细胞系在不添加白细胞介素-2（IL-2）的情况下持续生长，并表达HTLV-I的病毒特异性蛋白和IL-2受体。接种自体细胞系后，在它们的血浆中可检测到针对HTLV-I蛋白的特异性抗体，并且在至少6个月的时间里可从它们的外周血中回收转化的HTLV-I感染细胞。然而，截至目前，即接种后2年，尚未观察到ATL的迹象。
