Nakamura H, Hayami M, Ohta Y, Ishikawa K, Tsujimoto H, Kiyokawa T, Yoshida M, Sasagawa A, Honjo S
Int J Cancer. 1987 Sep 15;40(3):403-7. doi: 10.1002/ijc.2910400320.
Protection against human T-cell leukemia virus type-I (HTLV-I) infection in cynomolgus monkeys, achieved by immunizing the animals with env gene products of HTLV-I produced in Escherichia coli, was evaluated. Four monkeys that had been immunized with the env product produced antibody against HTLV-I gp68 and gp46, and their sera were found to cause strong inhibition of syncytium formation of a cat fibroblast cell line induced by HTLV-I. Immunized and non-immunized monkeys were challenged with live MT-2 cells, a high HTLV-I-producer cell line. After challenge, all the control non-immunized monkeys were infected with HTLV-I, as judged by the frequent detection of HTLV-I-antigens in cultures of their peripheral blood mononuclear cells (PBMC), whereas no antigens were recovered from PBMC of immunized monkeys. These results indicate that humoral immunity against HTLV-I-envelope protein elicited by immunization with the polypeptides synthesized in bacteria protected the monkeys against primary infection with HTLV-I.
通过用在大肠杆菌中产生的人类嗜T细胞病毒I型（HTLV-I）的env基因产物免疫食蟹猴，评估其对HTLV-I感染的保护作用。四只用env产物免疫的猴子产生了针对HTLV-I gp68和gp46的抗体，并且发现它们的血清对由HTLV-I诱导的猫成纤维细胞系的合胞体形成有强烈抑制作用。用高HTLV-I产生细胞系活MT-2细胞对免疫和未免疫的猴子进行攻击。攻击后，通过在其外周血单个核细胞（PBMC）培养物中频繁检测到HTLV-I抗原判断，所有对照未免疫的猴子都感染了HTLV-I，而从免疫猴子的PBMC中未检测到抗原。这些结果表明，用细菌合成的多肽免疫引发的针对HTLV-I包膜蛋白的体液免疫保护猴子免受HTLV-I的原发性感染。
