Xie Dafei, Yuan Peiwen, Wang Dong, Jin Hua, Chen Hui
Department of General Surgery, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China.
Department of Intensive Care Unit, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China.
Oncol Lett. 2017 Aug;14(2):1455-1459. doi: 10.3892/ol.2017.6278. Epub 2017 May 29.
The effects of naringin on the expression of miR-19b and cell apoptosis were investigated in the human hepatocellular carcinoma cell line HepG2. HepG2 cells were treated with varied concentrations of naringin. The effects of naringin on the proliferation of HepG2 cells were observed by an MTT assay, morphological changes of cells were observed by an inverted microscope, cell apoptosis was detected by DAPI staining, miR-19b mRNA levels were determined with RT-PCR, and the expression of Bax and Bcl-2 proteins was examined by western blot assay. MTT results showed that naringin significantly inhibited the proliferation of HepG2 cells. Apoptotic HepG2 cells showed obvious changes in morphology under inverted microscope. DAPI staining suggested that naringin could induce cell shrinkage and nuclear chromatin condensation. RT-PCR results showed that naringin could upregulate the expression of miR-19b mRNA. Finally, western blot suggested that naringin upregulated the expression of Bax protein, but downregulated the expression of Bcl-2 protein. In conclusion, naringin can upregulate the expression of miR-19b mRNA and induce HepG2 cell apoptosis. In addition, it can also upregulate the expression of Bax protein and downregulate the expression of Bcl-2 protein during the process of apoptosis.
在人肝癌细胞系HepG2中研究了柚皮苷对miR-19b表达和细胞凋亡的影响。用不同浓度的柚皮苷处理HepG2细胞。通过MTT法观察柚皮苷对HepG2细胞增殖的影响，用倒置显微镜观察细胞形态变化，通过DAPI染色检测细胞凋亡，用RT-PCR测定miR-19b mRNA水平，并用蛋白质印迹法检测Bax和Bcl-2蛋白的表达。MTT结果表明，柚皮苷显著抑制HepG2细胞的增殖。凋亡的HepG2细胞在倒置显微镜下显示出明显的形态变化。DAPI染色表明柚皮苷可诱导细胞收缩和核染色质浓缩。RT-PCR结果表明，柚皮苷可上调miR-19b mRNA的表达。最后，蛋白质印迹法表明柚皮苷上调了Bax蛋白的表达，但下调了Bcl-2蛋白的表达。总之，柚皮苷可上调miR-19b mRNA的表达并诱导HepG2细胞凋亡。此外，在凋亡过程中它还可上调Bax蛋白的表达并下调Bcl-2蛋白的表达。
