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用于多种肽能神经元电子显微镜检查的差异免疫标记

Differential immunolabeling for electron microscopy of diverse peptidergic neurons.

作者信息

Maegawa M, Hisano S, Tsuruo Y, Katoh S, Nakanishi J, Chikamori-Aoyama M, Daikoku S

出版信息

J Histochem Cytochem. 1987 Feb;35(2):251-5. doi: 10.1177/35.2.2878952.

DOI:10.1177/35.2.2878952
PMID:2878952
Abstract

We describe a simple and reliable method for differential immunolabeling of pre- and post-synaptic signal peptides at the ultrastructural level. Hypothalamic tissues of rats, including the suprachiasmatic nucleus, were cut on a Vibratome. Visualization of the immunolabeling of somatostatin (SRIH) and vasoactive intestinal polypeptide (VIP) was performed with avidin-biotin-peroxidase-diaminobenzidine (DAB). The end product of the DAB to VIP was further silver-intensified in a physical processing using silver nitrate, and the silver grains were finally substituted for gold. DAB-labeled SRIH fibers synapse on gold-labeled VIP perikarya and dendrites in the suprachiasmatic nucleus.

摘要

我们描述了一种在超微结构水平上对突触前和突触后信号肽进行差异免疫标记的简单可靠方法。将包括视交叉上核在内的大鼠下丘脑组织在振动切片机上切割。使用抗生物素蛋白-生物素-过氧化物酶-二氨基联苯胺（DAB）对生长抑素（SRIH）和血管活性肠肽（VIP）进行免疫标记可视化。DAB与VIP的终产物在使用硝酸银的物理处理中进一步进行银增强，最后银颗粒被金取代。在视交叉上核中，DAB标记的SRIH纤维与金标记的VIP核周体和树突形成突触。

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1
Differential immunolabeling for electron microscopy of diverse peptidergic neurons.用于多种肽能神经元电子显微镜检查的差异免疫标记
J Histochem Cytochem. 1987 Feb;35(2):251-5. doi: 10.1177/35.2.2878952.
2
Immunohistochemical evidence of serotoninergic regulation of vasoactive intestinal polypeptide (VIP) in the rat suprachiasmatic nucleus.大鼠视交叉上核中血清素能对血管活性肠肽（VIP）调节的免疫组织化学证据。
Histochemistry. 1987;86(6):573-8. doi: 10.1007/BF00489549.
3
Suprachiasmatic nucleus neurons immunoreactive for vasoactive intestinal polypeptide have synaptic contacts with axons immunoreactive for neuropeptide Y: an immunoelectron microscopic study in the rat.视交叉上核中对血管活性肠多肽免疫反应阳性的神经元与对神经肽Y免疫反应阳性的轴突有突触联系：大鼠的免疫电子显微镜研究
Neurosci Lett. 1988 May 26;88(2):145-50. doi: 10.1016/0304-3940(88)90116-4.
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Synaptic relationships between neurons containing vasopressin, gastrin-releasing peptide, vasoactive intestinal polypeptide, and glutamate decarboxylase immunoreactivity in the suprachiasmatic nucleus: dual ultrastructural immunocytochemistry with gold-substituted silver peroxidase.视交叉上核中含有加压素、胃泌素释放肽、血管活性肠多肽和谷氨酸脱羧酶免疫反应性的神经元之间的突触关系：金取代银过氧化物酶双超微结构免疫细胞化学
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[Distribution of peptidergic neurons in the rat suprachiasmatic nucleus].
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Immunohistochemical evidence for synaptic connections between neuropeptide Y-containing axons and periventricular somatostatin neurons in the anterior hypothalamus in rats.大鼠下丘脑前部含神经肽Y的轴突与室周生长抑素神经元之间突触连接的免疫组织化学证据。
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Neuronal associations in the rat suprachiasmatic nucleus demonstrated by immunoelectron microscopy.
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Catecholaminergic innervation of the suprachiasmatic nucleus in the adult rat: ultrastructural relationships with neurons containing vasoactive intestinal peptide or vasopressin.成年大鼠视交叉上核的儿茶酚胺能神经支配：与含血管活性肠肽或加压素神经元的超微结构关系
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Immunocytochemical localization of vasoactive intestinal polypeptide-containing cells and processes in the suprachiasmatic nucleus of the rat: light and electron microscopic analysis.大鼠视交叉上核中含血管活性肠肽的细胞及突起的免疫细胞化学定位：光镜和电镜分析
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The direct retinal projection to VIP neuronal elements in the rat SCN.
Brain Res Bull. 1993;31(6):637-40. doi: 10.1016/0361-9230(93)90134-w.

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Somatostatin regulates central clock function and circadian responses to light.生长抑素调节中枢时钟功能和对光的昼夜反应。
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2
Mutant vasopressin precursor in the endoplasmic reticulum of the Brattleboro rat. Ultrastructural evidence from individual "vasopressin" cells localized with the light microscope by use of a new gold/silver method for immunostain enhancement.
Cell Tissue Res. 1988 Sep;253(3):671-6. doi: 10.1007/BF00219759.
3
Immunohistochemical evidence of serotoninergic regulation of vasoactive intestinal polypeptide (VIP) in the rat suprachiasmatic nucleus.大鼠视交叉上核中血清素能对血管活性肠肽（VIP）调节的免疫组织化学证据。
Histochemistry. 1987;86(6):573-8. doi: 10.1007/BF00489549.
4
Synaptic regulation of paraventricular arginine vasopressin-containing neurons by neuropeptide Y-containing monoaminergic neurons in rats. Electron-microscopic triple labeling.大鼠中含神经肽Y的单胺能神经元对室旁含精氨酸加压素神经元的突触调节。电子显微镜三重标记
Cell Tissue Res. 1989 Aug;257(2):269-78. doi: 10.1007/BF00261830.
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Axons containing neuropeptide Y innervate arginine vasopressin-containing neurons in the rat paraventricular nucleus. Dual electron microscopic immunolabeling.含有神经肽Y的轴突支配大鼠室旁核中含精氨酸加压素的神经元。双重电子显微镜免疫标记。
Histochemistry. 1989;91(4):273-81. doi: 10.1007/BF00493001.
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Ultrastructural localization of immunolabeled substance P and methionine-enkephalin-octapeptide in the surface layer of the dorsal horn of rat spinal cord.大鼠脊髓背角表层中免疫标记的P物质和甲硫氨酸脑啡肽八肽的超微结构定位
Cell Tissue Res. 1988 Jul;253(1):55-60. doi: 10.1007/BF00221739.