Tanaka M, Ichitani Y, Okamura H, Tanaka Y, Ibata Y
Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
Brain Res Bull. 1993;31(6):637-40. doi: 10.1016/0361-9230(93)90134-w.
The connection between optic nerve terminals and vasoactive intestinal peptide (VIP)-containing neurons in the rat suprachiasmatic nucleus (SCN) was investigated using double labeling immunocytochemistry. Cholera toxin B subunit (CT) was injected into the right eyeball, and coronal sections of the SCN were then processed for CT immunocytochemistry with silver-gold intensification and VIP immunocytochemistry consecutively with DAB staining alone. In the ventrolateral portion of the SCN, CT immunoreactive (CT-IR) optic nerve terminals were found to make synaptic contacts not only with VIP-like immunoreactive (VIP-LI) dendrites, but also with VIP-LI perikarya. CT-IR nerve endings were also found to make synaptic contacts with non-VIP immunoreactive neuronal dendrites.
