Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China, Chengdu, 610041, China.
Department of Pathology, The First Affiliated Hospital of Jiujiang University, Jiujiang, 332005, China.
Sci Rep. 2017 Aug 8;7(1):7528. doi: 10.1038/s41598-017-07564-3.
Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples following Papanicolaou's or Special staining with Grocott's methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n = 54) and sputum samples (n = 117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT. LCT slides were generally superior to conventional slides, showing smaller cell monolayer surface area, clearer background and more distinct stereoscopic cytological features. For Papanicolaou's staining, LCT-prepared slides allowed a higher positive rate of Aspergillus identification than conventional slides for bronchial brushing samples (59.25% vs. 20.37%, P < 0.05) and sputum samples (29.05% vs. 8.55%, P < 0.05). Similarly, Special staining of LCT-prepared slides showed a higher positive rate of Aspergillus identification for bronchial brushing samples (83.33% vs. 57.41%, P < 0.05) and sputum samples (43.59% vs. 19.66%, P < 0.05). This preliminary study suggests that LCT may be better than conventional slide preparation for identifying Aspergillus in respiratory samples from patients with pulmonary aspergillosis.
传统的呼吸道样本细胞学检查有助于识别曲霉菌，但敏感性较差，因此本研究旨在评估液基细胞学检测（LCT）在巴氏染色或特殊染色（包括过碘酸雪夫染色和六胺银染色）后提高呼吸道样本中曲霉菌识别能力的潜力。收集了 171 例肺部曲霉菌病患者的支气管刷检样本（n=54）和痰液样本（n=117），分别采用传统细胞学或液基SurePath LCT 制作细胞学涂片。与传统涂片相比，LCT 涂片通常具有更小的细胞单层表面积、更清晰的背景和更明显的立体细胞学特征。对于巴氏染色，LCT 制备的涂片对支气管刷检样本（59.25% vs. 20.37%，P<0.05）和痰液样本（29.05% vs. 8.55%，P<0.05）的曲霉菌阳性识别率高于传统涂片。同样，LCT 制备的特殊染色涂片对支气管刷检样本（83.33% vs. 57.41%，P<0.05）和痰液样本（43.59% vs. 19.66%，P<0.05）的曲霉菌阳性识别率更高。这项初步研究表明，与传统涂片相比，LCT 可能更有助于识别肺部曲霉菌病患者的呼吸道样本中的曲霉菌。
